siRNA targeting KRAS
View Patent ↗Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rationale design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes.
1. An siRNA molecule, consisting of: (a) a sense region and an antisense region, wherein said sense region and said antisense region together form a duplex region consisting of 19-30 base pairs and said antisense region comprises a sequence that is the complement of SEQ. ID. NO. 461; and (b) from zero to four overhang regions, wherein each overhang region is six or fewer nucleotides in length.
2. The siRNA molecule of claim 1 , wherein said antisense region and said sense region are each 19-25 nucleotides in length.
3. The siRNA molecule of claim 2 , wherein said antisense region and said sense region are each 19 nucleotides in length.
4. The siRNA molecule of claim 1 , wherein said antisense region is 100% complementary to a region of a target mRNA.
5. The siRNA molecule of claim 1 , wherein said siRNA molecule has at least one overhang region.
6. The siRNA molecule of claim 1 , wherein said siRNA molecule has no overhang regions.
7. The siRNA molecule of claim 2 , wherein said siRNA molecule has at least one overhang region.
8. The siRNA molecule of claim 2 , wherein said siRNA molecule has no overhang regions.
9. The siRNA molecule of claim 3 , wherein said siRNA molecule has at least one overhang region.
10. The siRNA molecule of claim 3 , wherein said siRNA molecule has no overhang regions.
11. A chemically synthesized double stranded siRNA molecule that directs cleavage of an mRNA via RNA interference, wherein:
a. each strand of said double stranded siRNA molecule is between 19 and 30 nucleotides in length; and
b. one strand of said double stranded siRNA molecule comprises a sequence that is the complement of SEQ. ID. NO. 461.
12. The chemically synthesized double siRNA molecule of claim 11 , wherein each strand of said double stranded siRNA molecule is 19 nucleotides in length.
13. An siRNA molecule, wherein said siRNA molecule consists of: (a) a sense region, wherein said sense region consists of a sequence that is SEQ. ID. NO. 461, and an antisense region, wherein said antisense region consists of a sequence that is the complement of SEQ. ID. NO. 461; and (b) from zero to four overhang regions, wherein each overhang region is six or fewer nucleotides in length.