siRNA targeting survivin
View Patent ↗Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rationale design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes.
1. An siRNA molecule, wherein said siRNA molecule consists of: (a) a duplex region, wherein said duplex region consists of a sense region and an antisense region, wherein said sense region and said antisense region are each 19-30 bases in length and said antisense region comprises a sequence that is the complement of SEQ ID NO: 451 or SEQ ID NO: 452; and (b) either no overhang regions or at least one overhang region, wherein each overhang region has six or fewer bases.
2. The siRNA molecule of claim 1 , wherein said antisense region and said sense region are each 19-25 nucleotides in length.
3. The siRNA molecule of claim 2 , wherein said antisense region and said sense region are each 19 nucleotides in length.
4. The siRNA molecule of claim 1 , wherein said siRNA molecule has at least one overhang region.
5. The siRNA molecule of claim 1 , wherein said siRNA molecule has no overhang regions.
6. The siRNA molecule of claim 2 , wherein said siRNA molecule has at least one overhang region.
7. The siRNA molecule of claim 2 , wherein said siRNA molecule has no overhang regions.
8. The siRNA molecule of claim 3 , wherein said siRNA molecule has at least one overhang region.
9. The siRNA molecule of claim 3 , wherein said siRNA molecule has no overhang regions.
10. The siRNA molecule of claim 1 , wherein said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 451.
11. The siRNA molecule of claim 1 , wherein said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 452.
12. A chemically synthesized double stranded siRNA molecule, wherein:
(a) each strand of said double stranded siRNA molecule is between 19 and 30 nucleotides in length; and
(b) one strand of said double stranded siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 451 or SEQ ID NO: 452.
13. The chemically synthesized double stranded siRNA molecule of claim 12 , wherein each strand of said double stranded siRNA molecule is 19-25 nucleotides in length.
14. The chemically synthesized double stranded siRNA molecule of claim 12 , wherein said one strand of said double stranded siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 451.
15. The chemically synthesized double stranded siRNA molecule of claim 12 , wherein said one strand of said double stranded siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 452.
16. A pool of at least two siRNAs, wherein said pool comprises a first siRNA and a second siRNA, wherein said first siRNA consists of a duplex region of 19-30 base pairs that has a first antisense region that comprises a sequence that is complementary to SEQ ID NO: 451 and either no overhang regions or at least one overhang region, wherein each overhang region has six or fewer nucleotides; and said second siRNA consists of a duplex region of 19-30 base pairs that has a second antisense region that comprises a sequence that is complementary to SEQ ID NO: 452 and either no overhang regions or at least one overhang region, wherein each overhang region has six or fewer nucleotides.
17. The pool of claim 16 , wherein said first siRNA and said second siRNA each have no overhangs.
18. The pool of claim 16 , wherein said duplex region of said first siRNA and said duplex region of said second siRNA are each 19-25 base pairs in length.
19. The pool of claim 16 , wherein said duplex region of said first siRNA and said duplex region of said second siRNA are each 19 base pairs in length.
20. The chemically synthesized double-stranded siRNA molecule of claim 13 , wherein each strand of said double-stranded siRNA molecule is 19 nucleotides in length.