Cartilage repair methods
View Patent ↗The present application discloses methods for repairing hyaline cartilage defects. The methods comprise a combination of introducing autologous bone mesenchymal stem cells to a joint, and applying to the joint a membrane comprising a polyester entangled with a polysaccharide. In some aspects, the bone mesenchymal stem cells are mesenchymal stem cells originating in bone underlying the joint. In these aspects, contact between the joint and the mesenchymal stem cells can be effected by introducing apertures through the bone using standard surgical techniques such as microfracture, abrasion, or drilling. Cartilage which forms in response to application of these methods is hyaline cartilage rather than fibrocartilage.
1. A method for repairing a full-thickness chondral defect of a joint of a patient in need of treatment, the method comprising:
a) microfracturing bone underlying the joint;
b) applying to the joint a membrane, wherein the membrane comprises a polyester polymer entangled with a hyaluronate polymer, at a weight ratio of polyester polymer to hyaluronate polymer of between 9:1 and 1:9, wherein the entanglement of the polymers is produced by forming an emulsified mixture of the polyester polymer in a first solvent and the hyaluronate polymer in a second solvent, adding the emulsified mixture to a mold, freezing the emulsified mixture in the mold, removing solvent from the resultant frozen mixture by vacuum, wherein the membrane has a thickness of at least 0.5 mm up to 3 mm; and
c) anchoring the membrane to the joint.
2. The method in accordance with claim 1 , for repairing a full-thickness chondral defect in a knee joint, an elbow joint, or a shoulder joint, wherein said microfracturing, applying and anchoring steps are carried out on the defective joint.
3. The method in accordance with claim 1 , wherein the polyester polymers comprise a co-polymer comprising polylactic acid and polyglycolic acid.
4. The method in accordance with claim 2 , wherein the polylactic acid and the polyglycolic acid are in a weight ratio of about 5:1 to about 2:1.
5. The method in accordance with claim 1 , wherein the anchoring the membrane to the joint comprises applying at least one fastener which secures the membrane to the joint, wherein the at least one fastener is selected from the group consisting of a biocompatible glue, a suture, a tissue weld, a dart, a staple, a screw, and a tack.
6. A method for repairing a full-thickness chondral defect of a joint of a patient in need of treatment, the method comprising:
a) introducing at least one aperture through bone underlying the joint, wherein the at least one aperture makes possible migration to the joint of mesenchymal stem cells comprised by a marrow cavity of the bone;
b) applying to the joint a membrane, wherein the membrane comprises a polyester polymer entangled with a hyaluronate polymer, at a weight ratio of polyester polymer to hyaluronate polymer of between 9:1 and 1:9, wherein the entanglement of the polymers is produced by forming an emulsified mixture of the polyester polymer in a first solvent and the hyaluronate polymer in a second solvent, adding the emulsified mixture to a mold, freezing the emulsified mixture in the mold, removing solvent from the resultant frozen mixture by vacuum, wherein the membrane has a thickness of at least 0.5 mm up to 3 mm; and
c) securing the membrane to the joint.
7. The method in accordance with claim 6 , wherein the polyester polymers comprise a co-polymer comprising polylactic acid and polyglycolic acid.
8. The method in accordance with claim 7 , wherein the polylactic acid and the polyglycolic acid are in a weight ratio of about 5:1 to about 2:1.
9. The method for repairing a hyaline cartilage defect in accordance with claim 6 , wherein the securing the membrane to the joint comprises applying at least one fastener to the membrane and the joint, wherein the at least one fastener is selected from the group consisting of a biocompatible glue, a suture, a tissue weld, a dart, a staple, a screw, and a tack.