Chondroitinase ABC I polynucleotides
View Patent ↗The invention relates to chondroitinase ABC I and uses thereof. In particular, the invention relates to recombinant and modified chondroitinase ABC I, their production and their uses. The chondroitinase ABC I enzymes of the invention are useful for a variety of purposes, including degrading and analyzing polysaccharides such as glycosaminoglycans (GAGs). These GAGs can include chondroitin sulfate, dermatan sulfate, unsulfated chondroitin and hyaluronan. The chondroitinase ABC I enzymes can also be used in therapeutic methods such as promoting nerve regeneration, promoting stroke recovery, treating spinal cord injury, treating epithelial disease, treating infections and treating cancer.
1. An isolated nucleic acid that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2.
2. The nucleic acid of claim 1 , wherein the nucleic acid sequence of the nucleic acid is as set forth in SEQ ID NO: 1.
3. A vector comprising the nucleic acid of claim 1 .
4. A vector comprising the nucleic acid of claim 2 .
5. A composition comprising the nucleic acid of claim 1 and a pharmaceutically acceptable carrier.
6. A composition comprising the nucleic acid of claim 2 and a pharmaceutically acceptable carrier.
7. A composition comprising the vector of claim 3 and a pharmaceutically acceptable carrier.
8. A composition comprising the vector of claim 4 and a pharmaceutically acceptable carrier.
9. A composition comprising the nucleic acid of claim 1 and a physiologically acceptable carrier.
10. A composition comprising the nucleic acid of claim 2 and a physiologically acceptable carrier.
11. A composition comprising the vector of claim 3 and a physiologically acceptable carrier.
12. A composition comprising the vector of claim 4 and a physiologically acceptable carrier.
13. An isolated nucleic acid that is a full-length complement of a nucleic acid encoding the polypeptide of SEQ ID NO: 2.
14. The isolated nucleic acid of claim 13 which is a full-length complement of the nucleic acid sequence of SEQ ID NO: 1.