Methods and compositions for the detection of a nucleic acid using a non-invasive cleavage reaction
View Patent ↗The invention provides methods, compositions and kits for generating a signal indicative of the presence of a target nucleic acid sequence in a sample comprising forming a cleavage structure by incubating a sample comprising a target nucleic acid sequence with upstream and downstream oligonucleotides, and cleaving the cleavage structure with a nuclease to generate a signal. The presence of a detectable signal is indicative of the presence of a target nucleic acid sequence and a non-invasive cleavage structure.
1. A method of detecting a target nucleic acid, comprising
(a) providing:
a target nucleic acid, which comprises in 3′ to 5′ order a first region and a second region,
a template nucleic acid, which comprises in 3′ to 5′ order a first region and a second region,
a first oligonucleotide that is at least partially complementary to said first region of said target nucleic acid,
a second oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is complementary to said second region of said target nucleic acid and wherein said 5′ region is not complementary to said target nucleic acid but is at least partially complementary to said first region of said template nucleic acid,
a third oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said template nucleic acid and said 5′ region is not complementary to said template nucleic acid,
a cleavage agent, and
a polymerization agent;
(b) mixing said target nucleic acid, said template nucleic acid, said first oligonucleotide, said second oligonucleotide, said third oligonucleotide, said cleavage agent and said polymerization agent under reaction conditions, wherein said reaction conditions permit:
forming a first duplex between said target nucleic acid and each of said fast oligonucleotide and said second oligonucleotide, wherein the 5′ region of the second oligonucleotide forms a first flap of a first non-invasive cleavage structure, wherein the complementary portions of said first oligonucleotide and said second oligonucleotide are separated by at least a nick when the fast duplex is formed,
cleaving the fast flap by said cleavage agent, thereby permitting release of the first flap,
forming a second duplex with the released fast flap, said template nucleic acid, and said third oligonucleotide, wherein the 5′ region of the third oligonucleotide forms a second flap of a second non-invasive cleavage structure, wherein the complementary portions of said first flap and said third oligonucleotide are separated by at least a nick when said second duplex is formed, and
cleaving the second flap by said cleavage agent; and
(c) detecting the non-invasive cleavage of the second flap, wherein detection of said non-invasive cleavage of said second flap is indicative of the presence of said target nucleic acid.
2. The method of claim 1 , wherein said first oligonucleotide has a single 3′ nucleotide that is non-complementary to said target nucleic acid.
3. The method of claim 1 , wherein said second and third oligonucleotide are resistant to invasive cleavage.
4. The method of claim 1 , wherein said released flap has a single 3′ nucleotide that is non-complementary to said template nucleic acid.
5. The method of claim 1 , wherein said first oligonucleotide has two or more 3′ nucleotides that are non-complementary to said target nucleic acid.
6. The method of claim 1 , wherein said first oligonucleotide and/or released flap comprise a 3′ block.
7. The method of claim 1 , wherein said released flap has two or more 3′ nucleotides that are non-complementary to said template nucleic acid.
8. The method of claim 1 , wherein said first and said second oligonucleotides are separated by a nick when said fast duplex is formed.
9. The method of claim 1 , wherein said first and said second oligonucleotides are separated by a 1 nucleotide gap when said first duplex is formed.
10. The method of claim 1 , wherein said first and second oligonucleotides are separated by a 2 nucleotide gap when said first duplex is formed.
11. The method of claim 1 , wherein said first and said second oligonucleotides are separated by a 3 nucleotide gap when said first duplex is formed.
12. The method of claim 1 , wherein said released flap and said third oligonucleotides are separated by a nick when said second duplex is formed.
13. The method of claim 1 , wherein said released flap and said third oligonucleotide are separated by a 1 nucleotide gap when said second duplex is formed.
14. The method of claim 1 , wherein said released flap and said third oligonucleotide are separated by a 2 nucleotide gap when said second duplex is formed.
15. The method of claim 1 , wherein said released flap and said third oligonucleotide are separated by a 3 nucleotide gap when said second duplex is formed.
16. The method of claim 1 , further comprising a forward primer complementary so the first region of said target nucleic acid.
17. The method of claim 16 , further comprising a reverse primer complementary to the second region of said target nucleic acid.
18. The method of claim 1 , wherein said polymerization agent lacks 5′ to 3′ exonuclease activity.
19. The method of claim 1 , wherein said polymerization agent is thermostable.
20. The method of claim 1 , wherein a single enzyme comprises said polymerization agent and said cleavage agent.
21. The method of claim 20 , wherein said enzyme is E. coli DNA polymerase I, T7 DNA polymerase, Tth DNA polymerase, or Taq DNA polymerase.
22. The method of claim 1 , wherein a first enzyme comprises said polymerization agent and a second enzyme comprises said cleavage agent.
23. The method of claim 1 , wherein said cleavage agent is a 5′ nuclease.
24. The method of claim 1 , wherein said cleavage agent is a FEN-1 nuclease.
25. The method of claim 23 , wherein said 5′ nuclease is thermostable.
26. The method of claim 22 , wherein said fast and second enzymes are provided in a single formulation.
27. The method of claim 22 , wherein said first enzyme is a Pfu DNA Polymerase and said second enzyme is a FEN-1 nuclease.
28. The method of claim 1 , wherein said third oligonucleotide comprises a pair of interactive labels.
29. The method of claim 1 , wherein a fast member of a pair of interactive labels is operatively coupled to the 5′ flap of said third oligonucleotide.
30. The method of claim 29 , wherein the second member of the pair of interactive labels is operatively coupled to position +1 of said third oligonucleotide.
31. The method of claim 29 , wherein the second member of the pair of interactive labels is operatively coupled to position +2 of said third oligonucleotide.
32. The method of claim 28 , wherein said pair of interactive labels comprises a fluorophore and a quencher.
33. The method of claim 32 , wherein said quencher is operatively coupled to position +1 of said third oligonucleotide and said fluorophore is operatively coupled to the 5′ flap of said third oligonucleotide.
34. The method of claim 32 , wherein said detection of a signal comprises detecting a change in fluorescent between said first and second members of said pair of interactive labels upon cleavage of said third oligonucleotide.
35. A method of detecting a target nucleic acid, comprising
(a) providing:
a target nucleic acid, which comprises in 3′ to 5′ order a first region and a second region,
a template nucleic acid, which comprises in 3′ to 5′ order a first region and a second region,
a fast oligonucleotide that is at least partially complementary to said first region of said target nucleic acid,
a second oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is complementary to said second region of said target nucleic acid and wherein said 5′ region is not complementary to said target nucleic acid but is at least partially complementary to said first region of said template nucleic acid,
a third oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said template nucleic acid and said 5′ region is not complementary to said template nucleic acid,
a cleavage agent, and
a polymerization agent;
(b) forming a reaction mixture by mixing said target nucleic acid, said first oligonucleotide, said second oligonucleotide, said cleavage agent and said polymerization agent under reaction conditions, wherein said reaction conditions permit:
forming of a fast duplex between said target nucleic acid and each of said first oligonucleotide and said second oligonucleotide, wherein the 5′ region of the second oligonucleotide forms a first flap of a first non-invasive cleavage structure, and wherein the complementary portions of said fast oligonucleotide and said second oligonucleotide are separated by at least a nick when said fast duplex is formed,
cleavage of the first flap by said cleavage agent, thereby permitting release of the first flap,
(c) mixing the released first flap, said third oligonucleotide, and said template nucleic acid in said reaction mixture under reaction conditions, wherein said reaction conditions permit:
forming of a second duplex with the released first flap, said template nucleic acid, and said third oligonucleotide, wherein the 5′ region of the third oligonucleotide forms a second flap of a second non-invasive cleavage structure, wherein the complementary portions of said first flap and said third oligonucleotide are separated by at least a nick when said second duplex is formed, and
cleaving of the second flap by said cleavage agent; and
(d) detecting the non-invasive cleavage of the second flap, wherein detection of said non-invasive cleavage of said second flap is indicative of the presence of said target nucleic acid.
36. A composition comprising:
a target nucleic acid, which comprises in 3′ to 5′ order a first region and a second region,
(ii) a template nucleic acid, which comprises in 3′ to 5′ order a first region and a second region,
(iii) a first oligonucleotide that is at least partially complementary to said first region of a said target nucleic acid;
(iv) a second oligonucleotide comprising a 5′ flap region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said target nucleic acid and wherein said 5′ flap region is non-complementary to said target nucleic acid but is at least partially complementary to said fast region of said template nucleic acid; and
(v) a third oligonucleotide comprising a 5′ flap region and a 3′ region, wherein said 3′ region is at least partially complementary to said second region of said template nucleic acid and said 5′ flap region is non-complementary to said template nucleic acid, and wherein a first member of a pair of interactive labels is operatively coupled to said 5′ flap region and wherein a second member of the pair of interactive labels is operatively coupled to the said 3′ region.
37. A method of detecting a target nucleic acid, comprising
(a) providing:
a target nucleic acid, which comprises in 3′ to 5′ order a first region and a second region,
a fast oligonucleotide that is at least partially complementary to said fast region of said target nucleic acid,
a second oligonucleotide comprising a 5′ region and a 3′ region, wherein said 3′ region is complementary to said second region of said target nucleic acid and wherein said 5′ region is not complementary to said target nucleic acid,
a cleavage agent, and
a polymerization agent;
(b) mixing said target nucleic acid, said first oligonucleotide, said second oligonucleotide, said cleavage agent and said polymerization agent under reaction conditions, wherein said reaction conditions permit:
forming a duplex between said target nucleic acid and each of said first oligonucleotide and said second oligonucleotide, wherein the 5′ region of the second oligonucleotide forms a flap of a non-invasive cleavage structure, wherein the complementary portions of said first oligonucleotide and said second oligonucleotide are separated by at least a nick when said duplex is formed,
cleaving the flap by said cleavage agent, and
(c) detecting the non-invasive cleavage of the flap, wherein detection of said non-invasive cleavage of said flap is indicative of the presence of said target nucleic acid.