Methods and compositions for promoting attachment of cells of endothelial cell lineage to medical devices
View Patent ↗The present invention provides compositions and methods for an improved coating for medical devices. Provided is a biofunctional coating composition comprising at least one binding domain that has binding specificity for a surface material of a medical device, and at least one binding domain that has binding specificity for cells of endothelial cell lineage. Methods for coating a surface of a medical device, and for manufacturing of a medical device, comprise contacting the surface to be coated with the biofunctional coating material in an amount effective to form a coating, and may further comprise contacting the coated surface with cells of endothelial cell lineage to bind the cells of endothelial cell lineage to the coated surface.
1. A method for coating a medical device, the method comprising contacting the medical device with a biofunctional coating composition to form a medical device having at least one coated surface; wherein the biofunctional coating composition comprises a surface-binding domain and an endothelial-cell binding domain; wherein the surface-binding domain comprises a peptide which binds to a material of which the medical device is comprised; wherein the endothelial-cell binding domain comprises a peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, and a combination thereof that binds to cells of endothelial cell lineage; and wherein the surface-binding domain and the endothelial-cell binding domain are coupled together.
2. The method according to claim 1 , wherein the medical device comprises a vascular device.
3. The method according to claim 2 , wherein the vascular device comprises a stent.
4. The method according to claim 1 , wherein the at least one surface-binding domain and the at least one endothelial-cell binding domain are coupled via a linker.
5. The method according to claim 1 , wherein the biofunctional coating composition comprises more than one type of surface-binding domain, and wherein each type of surface-binding domain is capable of binding to the surface material of the medical device.
6. The method according to claim 1 , wherein the biofunctional coating composition comprises more than one type of endothelial-cell binding domain, and wherein each type of endothelial-binding domain has a binding specificity for cells of endothelial cell lineage that differs from another type of endothelial-binding domain present in the biofunctional coating composition.
7. A medical device comprising at least one surface of the medical device coated with a biofunctional coating composition, wherein the biofunctional coating composition comprises at least one surface-binding domain and at least one endothelial-cell binding domain; wherein a surface-binding domain comprises a peptide which binds to a material comprising a material selected from the group consisting of metal, metal oxide, non-metal oxide, ceramic, polymer, and a combination thereof; wherein an endothelial-cell binding domain comprises a peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, and a combination thereof that binds to cells of endothelial cell lineage; and wherein the at least one surface-binding domain and the at least one endothelial-cell binding domain are coupled together.
8. The medical device according to claim 7 , wherein the medical device comprises a vascular device.
9. The medical device according to claim 8 , wherein the vascular device comprises a stent.
10. The medical device according to claim 7 , wherein the at least one surface-binding domain and the at least one endothelial-cell binding domain are coupled via a linker.
11. The medical device according to claim 7 , wherein the biofunctional coating composition comprises more than one type of surface-binding domain, and wherein each type of surface-binding domain is capable of binding to the surface material of the medical device.
12. The medical device according to claim 7 , wherein the biofunctional coating composition comprises more than one type of endothelial-binding domain, and wherein each type of endothelial-binding domain has a binding specificity for cells of endothelial cell lineage that differs from another type of endothelial-binding domain present in the biofunctional coating composition.
13. The medical device according to claim 7 , further comprising cells of endothelial cell lineage.
14. A medical device comprising a biofunctional coating composition that promotes attachment of endothelial cells, wherein the biofunctional coating composition comprises a surface-binding domain coupled to an endothelial cell-binding domain, wherein the surface-binding domain comprises a peptide which binds to a material of which the medical device is comprised, and wherein an endothelial-cell binding domain comprises a peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, and a combination thereof that binds to cells of endothelial cell lineage.
15. The medical device according to claim 14 , wherein the medical device comprises a vascular device.
16. The medical device according to claim 14 , wherein the vascular device comprises a stent.
17. The medical device according to claim 14 , wherein the surface-binding domain and the endothelial-binding domain are coupled via a linker.
18. The medical device according to claim 14 , further comprising cells of endothelial cell lineage.
19. The medical device according to claim 14 , wherein the biofunctional coating composition is multimeric.
20. The method according to claim 1 , further comprising contacting the biofunctional coating composition with cells of endothelial cell lineage.
21. The method according to claim 1 , wherein the material comprises a material selected from the group consisting of metal, metal oxide, non-metal oxide, ceramic, polymer, and a combination thereof.
22. The method according to claim 1 , wherein the biofunctional coating composition is multimeric.