Affinity chromatography matrices and methods of making and using the same
View Patent ↗The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.
1. A method of coupling a protein ligand to a solid support comprising: a) contacting the solid support with an associative group such that the associative group interacts with the solid support; b) activating the solid support using an activating group; and c) contacting the solid support with the protein ligand such that the protein ligand binds to the activated solid support and non-covalently interacts with the associative group of a), wherein the associative group and the activating group are both linked separately to the solid support.
2. The method of claim 1 , wherein the solid support is a bead.
3. The method of claim 2 , wherein the bead is an agarose bead.
4. The method of claim 1 , wherein the associative group is a charged species.
5. The method of claim 4 , wherein the charged species bears a positive charge.
6. The method of claim 4 , wherein the charged species bears a negative charge.
7. The method of claim 1 , wherein the associative group is hydrophobic.
8. The method of claim 1 , wherein the solid support is activated by contacting the solid support with at least one activating group selected from the following: a) cyanogen bromide; b) an aldehyde; c) an epoxide; d) an activated carboxylic acid.
9. The method of claim 1 , wherein the protein ligand also can bind an immunoglobulin.
10. The method of claim 9 , wherein the protein ligand is chosen from protein A and protein G.
11. The method of claim 1 , wherein the protein ligand covalently binds to the solid support.
12. The method of claim 1 , wherein the interaction between the associative group and the solid support is a covalent bond.
13. The method of claim 1 , wherein the separate linking of the associative group and the activating group to the solid support comprises separating the reactions for binding of the associative group and the activating group to the solid support.