Isolation and purification of hematopoietic stem cells from post-liposuction lipoaspirates

1. A method for isolating a CD34 + cell, the method comprising:

obtaining adipose tissue,

preparing a stromal vascular fraction (SVF) from the adipose tissue;

incubating SVF cells in a stromal cell media in a cell culture apparatus for a time suitable for the culture of cells in vitro to allow the cells to adhere to the culture apparatus; separating nonadherent cells in the SVF from adherent cells in the SVF; and

identifying CD34+ cells in the non-adherent cell population, and isolating the CD34 + cells from the CD34− nonadherent cells,

wherein at least about 40% of the non-adherent cells are CD34 + cells.

2. The method of claim 1 , wherein the CD34 + cell is isolated in the ratio of at least about 1×10 7 CD34 + cells isolated per about 100 ml of adipose tissue.

3. The method of claim 1 , wherein the obtaining step comprises lipoaspiration.

4. The method of claim 1 , wherein the adipose tissue is human.

5. The method of claim 1 , wherein the CD34 + cell is selected from the group consisting of a CD34 + SSC lo cell, a CD34 + CD45 + cell, a CD34 hi CD38 − CD4 − cell, a CD34 + CD38 − CD41 − SSC lo , a CD34 lo CD45 − cell, a CD34 lo CD45 + cell, a CD34 + ALDH br cell and a CD34 + ALDH br SSC lo cell.

6. The method of claim 1 , further comprising the step of isolating a CD34 hi cell or a CD34 lo cell.

7. The method of claim 1 , wherein the CD34 + cell is selected from the group consisting a colony-forming unit granulocyte macrophage (CFU-GM), a burst-forming unit erythrocyte (BFU-E) and a colony-forming unit granulocyte erythrocyte macrophage megakaryocyte (CFU-GEMM).

8. The method of claim 1 , further comprising harvesting the adherent cells, thereby harvesting adipose tissue-derived stromal cells.