Device and method for multiple analyte detection
View Patent ↗The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
1. A device comprising:
a substrate;
a sample-distribution network defined by the substrate and comprising (i) a sample inlet, (ii) two or more detection chambers, and (iii) a fluid communication between the two or more detection chambers and the inlet; and
non-immobilized detection reagents pre-loaded in each of the two or more detection chambers, the detection reagents comprising a first oligonucleotide, a second oligonucleotide, and a detection reagent capable of producing an optical signal;
wherein the fluid communication comprises a channel and respective fluid connections connecting the channel to the respective detection chambers of the two or more detection chambers, and the fluid connections branch off from opposite sides of the channel.
2. The device of claim 1 , wherein the fluid connections branch off in a staggered manner from opposite sides of the channel.
3. The device of claim 2 , wherein each fluid connection comprises an L-shaped configuration.
4. The device of claim 1 , wherein the fluid connections branch off in pairs of opposing fluid connections on opposite sides of the channel.
5. The device of claim 4 , wherein each fluid connection comprises an L-shaped configuration.
6. The device of claim 1 , wherein the detection reagents have been dried down in the two or more detection chambers.
7. The device of claim 1 , wherein the first oligonucleotide is effective to selectively bind to a target polynucleotide having a defined sequence.
8. The device of claim 1 , wherein the first oligonucleotide and the second oligonucleotide comprise an oligonucleotide primer pair suitable for amplifying, by polymerase chain reaction, a selected target polynucleotide region.
9. The device of claim 1 , wherein the substrate comprises a copolymer material.
10. The device of claim 1 , wherein the substrate further comprises a high thermal conductivity metal surface.
11. The device of claim 10 , wherein the high thermal conductivity metal surface comprises copper.
12. The device of claim 10 , wherein the high thermal conductivity metal surface comprises aluminum.
13. The device of claim 10 , further comprising means for modulating the temperature of the two or more detection chambers, in the substrate.
14. The device of claim 1 , further comprising an optically clear cover layer disposed over the sample distribution network.
15. The device of claim 1 , wherein the detection reagent capable of producing an optical signal comprises a fluorescently detectable detection reagent.
16. The device of claim 1 , wherein the substrate defines at least two such sample-distribution networks.
17. The device of claim 1 , further comprising means for modulating the temperature of the two or more detection chambers, in the substrate.
18. The device of claim 1 , wherein the substrate comprises two or more layers.
19. The device of claim 18 , wherein the two or more layers are laminated together.
20. The device of claim 18 , wherein the two or more layers comprise at least one silicon layer.
21. The device of claim 20 , further comprising an oxidation layer coated on the at least one silicon layer, wherein each of the two or more detection chambers is at least partially defined by the oxidation layer and the oxidation layer separates the at least one silicon layer from each of the two or more detection chambers.
22. A system comprising the device of claim 1 and an external temperature controller.
23. The device of claim 1 , wherein the fluid communication comprises a sample delivery channel, and the device further comprises a plurality of dead-end fluid connections, wherein each of the dead-end fluid connections provides a dead-end communication between a respective one of the two or more detection chambers and the sample delivery channel.
24. A device comprising:
a substrate;
a sample-distribution network defined by the substrate and comprising (i) a sample inlet, (ii) two or more detection chambers, and (iii) a fluid communication between the two or more detection chambers and the inlet; and
detection reagents pre-loaded in each of the two or more detection chambers;
wherein the fluid communication comprises a channel and respective fluid connections connecting the channel to the respective detection chambers of the two or more detection chambers, the fluid connections branch off from opposite sides of the channel, the fluid communication comprises a sample delivery channel, and the device further comprises a plurality of dead-end fluid connections, wherein each of the dead-end fluid connections provides a dead-end communication between a respective one of the two or more detection chambers and the sample delivery channel.
25. The device of claim 24 , wherein the fluid connections branch off in a staggered manner from opposite sides of the channel.
26. The device of claim 25 , wherein each fluid connection comprises an L-shaped configuration.
27. The device of claim 25 , wherein the fluid connections branch off in pairs of opposing fluid connections on opposite sides of the channel.
28. The device of claim 27 , wherein each fluid connection comprises an L-shaped configuration.
29. The device of claim 24 , wherein the detection reagents have been dried down in the two or more detection chambers.
30. The device of claim 24 , wherein the detection reagents comprise a sequence-selective reagent.
31. The device of claim 30 , wherein the sequence-selective reagent comprises a polynucleotide effective to selectively bind to a target polynucleotide having a defined sequence.
32. The device of claim 24 , wherein the detection reagents comprise an oligonucleotide primer pair suitable for amplifying, by polymerase chain reaction, a selected target polynucleotide region.
33. The device of claim 24 , wherein the substrate comprises a copolymer material.
34. The device of claim 24 , wherein the substrate further comprises a high thermal conductivity metal surface.
35. The device of claim 34 , wherein the high thermal conductivity metal surface comprises copper.
36. The device of claim 34 , wherein the high thermal conductivity metal surface comprises aluminum.
37. The device of claim 34 , further comprising means for modulating the temperature of the two or more detection chambers, in the substrate.
38. The device of claim 24 , further comprising an optically clear cover layer disposed over the sample distribution network.
39. The device of claim 24 , wherein the detection reagents comprise fluorescently detectable detection reagents.
40. The device of claim 24 , wherein the substrate defines at least two such sample-distribution networks.
41. The device of claim 24 , further comprising means for modulating the temperature of the two or more detection chambers, in the substrate.
42. The device of claim 24 , wherein the substrate comprises two or more layers.
43. The device of claim 42 , wherein the two or more layers are laminated together.
44. The device of claim 42 , wherein the two or more layers comprise at least one silicon layer.
45. The device of claim 44 , further comprising an oxidation layer coated on the at least one silicon layer, wherein each of the two or more detection chambers is at least partially defined by the oxidation layer and the oxidation layer separates the at least one silicon layer from each of the two or more detection chambers.