Device and method for multiple analyte detection
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
1 . A device comprising:
a substrate defining a sample-distribution network comprising
a sample inlet,
a plurality of detection chambers, and
a channel providing fluid communication between each of the plurality of detection chambers and the sample inlet; and
a detection reagent comprising enzyme substrate molecules designed to react with a selected analyte enzyme and pre-loaded in each of the plurality of detection chambers.
2 . The device of claim 1 , wherein the enzyme substrate molecules comprise a chromogenic substrate.
3 . The device of claim 2 , wherein the chromogenic substrate comprises a reducing dye.
4 . The device of claim 2 , wherein the chromogenic substrate is at least one of NAD/NADH, FAD/FADH, and nitrophenol.
5 . The device of claim 1 , wherein the detection reagent further comprises a carrier protein.
6 . The device of claim 5 , wherein the carrier protein comprises bovine serum albumin.
7 . The device of claim 1 , wherein the enzyme substrate molecules have been dried down.
8 . The device of claim 1 , wherein one or more detection chambers of the plurality of detection chambers comprise a control.
9 . The device of claim 8 , wherein the control comprises a selected reagent.
10 . The device of claim 1 , wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a selected reagent therein, and the selected reagent emits light at a second wavelength that is distinctive from each first respective wavelength.
11 . The device of claim 1 , wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein, and the calibration standard emits light at a second wavelength that is distinctive from each first respective wavelength.
12 . The device of claim 1 , wherein each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein.
13 . A device comprising:
a substrate defining a sample-distribution network comprising
a sample inlet,
a plurality of detection chambers, and
a channel providing fluid communication between each of the plurality of detection chambers and the sample inlet; and
a detection reagent comprising drug target molecules pre-loaded in each of the plurality of detection chambers and designed to react with respective selected analyte drugs.
14 . The device of claim 13 , wherein the drug target molecules comprise a receptor protein.
15 . The device of claim 13 , wherein the drug target molecules pre-loaded in each of the plurality of detection chambers are designed to react with at least one of a receptor protein agonist and a receptor protein antagonist.
16 . The device of claim 13 , wherein the drug target molecules pre-loaded in each of the plurality of detection chambers are designed to react with at least one of a substrate, an activator, and an inhibitor of a drug target.
17 . The device of claim 13 , wherein the detection reagent further comprises a carrier protein.
18 . The device of claim 17 , wherein the carrier protein comprises bovine serum albumin.
19 . The device of claim 13 , wherein the drug target molecules have been dried down.
20 . The device of claim 13 , wherein one or more detection chambers of the plurality of detection chambers comprise a control.
21 . The device of claim 20 , wherein the control comprises a selected reagent.
22 . The device of claim 13 , wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a selected reagent therein, and the selected reagent emits light at a second wavelength that is distinctive from each first respective wavelength.
23 . The device of claim 13 , wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein, and the calibration standard emits light at a second wavelength that is distinctive from each first respective wavelength.
24 . The device of claim 13 , wherein each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein.
25 . A device comprising:
a substrate defining a sample-distribution network comprising
a sample inlet,
a plurality of detection chambers, and
a channel providing fluid communication between each of the plurality of detection chambers and the sample inlet; and
thermally stable DNA polymerase pre-loaded in each of the plurality of detection chambers.
26 . The device of claim 25 , further comprising selected-sequence polynucleotides pre-loaded in each of the plurality of detection chambers.
27 . The device of claim 25 , wherein the thermally stable DNA polymerase has been dried down.
28 . The device of claim 25 , wherein one or more detection chambers of the plurality of detection chambers comprise a control.
29 . The device of claim 28 , wherein the control comprises a selected reagent.
30 . The device of claim 25 , wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a selected reagent therein, and the selected reagent emits light at a second wavelength that is distinctive from each first respective wavelength.
31 . The device of claim 25 , wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein, and the calibration standard emits light at a second wavelength that is distinctive from each first respective wavelength.
32 . The device of claim 25 , wherein each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein.
33 . A device comprising:
a substrate defining a sample-distribution network comprising
a sample inlet,
a plurality of detection chambers, and
a channel providing fluid communication between each of the plurality of detection chambers and the sample inlet; and
a pathogen detection reagent pre-loaded in each of the plurality of detection chambers and designed to detect a pathogen.
34 . The device of claim 33 , wherein one or more detection chambers of the plurality of detection chambers comprise a control.
35 . The device of claim 34 , wherein the control comprises a selected reagent.
36 . The device of claim 33 , wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a selected reagent therein, and the selected reagent emits light at a second wavelength that is distinctive from each first respective wavelength.
37 . The device of claim 33 , wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein, and the calibration standard emits light at a second wavelength that is distinctive from each first respective wavelength.
38 . The device of claim 33 , wherein one or more detection chambers of the plurality of detection chambers comprise a calibration standard.
39 . A method comprising:
providing a device, the device comprising
a substrate defining a sample-distribution network comprising
a sample inlet,
a plurality of detection chambers, and
a channel providing fluid communication between each of the plurality of detection chambers and the sample inlet, and
a pathogen detection reagent pre-loaded in each of the plurality of detection chambers and designed to detect a pathogen;
loading a sample in the detection chambers; and
detecting a pathogen in the detection chambers.
40 . The method of claim 39 , wherein one or more detection chambers of the plurality of detection chambers comprise a control, and the method further comprises detecting a signal generated by the control.
41 . The method of claim 40 , wherein the control comprises a selected reagent.
42 . The method of claim 39 , wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a selected reagent therein, the selected reagent emits light at a second wavelength that is distinctive from each first respective wavelength, and the method further comprises detecting the second wavelength and each first respective wavelength.
43 . The method of claim 39 , wherein the detection reagents in each chamber emit light at a first respective wavelength, each of the detection chambers of the plurality of detection chambers comprises a calibration standard therein, the calibration standard emits light at a second wavelength that is distinctive from each first respective wavelength, and the method further comprises detecting the second wavelength and each first respective wavelength.
44 . The method of claim 39 , wherein one or more detection chambers of the plurality of detection chambers comprise a calibration standard.