Method for attachment of silylated molecules to glass surfaces
View Patent ↗A method for the efficient immobilization of silylated molecules such as silylated oligonucleotides or proteins onto unmodified surfaces such as a glass surface is provided. Also provided are compounds, devices, and kits for modifying surfaces such as glass surfaces.
1. A compound having a formula vii:
[(R 1 )(R 2 )(R 3 )Si—X-Z-CYNH] 2 Si (NHCYL-M) 2 vii
wherein R 1 , R 2 and R 3 independently represents C 1 -C 6 alkoxy, C 1 -C 6 alkyl, phenyl, or aryl substituted with one or more groups selected from the group consisting of C 1 -C 6 alkyl and C 1 -C 6 alkoxy; L represents a linking group; X represents linear or branched C 1 -C 20 alkyl or aryl substituted with one or more groups selected from the group consisting of C 1 -C 6 alkyl and C 1 -C 6 alkoxy, optionally substituted with one or more heteroatoms comprising oxygen, nitrogen, or sulfur; Y represents oxygen or sulfur; and Z represents oxygen or NH; and M represents a molecule, with the proviso that at least one of R 1 , R 2 , or R 3 represents C 1 -C 6 alkoxy.
2. The compound of claim 1 wherein L represents a nucleophilic group from the molecule.
3. The compound of claim 2 wherein the nucleophilic group comprises —NH, —S—, —O—, or —OOC—.
4. A kit comprising a compound having a formula vii:
[(R 1 )(R 2 )(R 3 )Si—X-Z-CYNH] 2 Si (NHCYL-M) 2 vii
wherein R 1 , R 2 and R 3 independently represents C 1 -C 6 alkoxy, C 1 -C 6 alkyl, phenyl, or aryl substituted with one or more groups selected from the group consisting of C 1 -C 6 alkyl and C 1 -C 6 alkoxy; L represents a linking group; X represents linear or branched C 1 -C 20 alkyl or aryl substituted with one or more groups selected from the group consisting of C 1 -C 6 alkyl and C 1 -C 6 alkoxy, optionally substituted with one or more heteroatoms comprising oxygen, nitrogen, or sulfur; Y represents oxygen or sulfur; and Z represents oxygen or NH; and M represents a molecule, with the proviso that at least one of R 1 , R 2, or R 3 represents C 1 -C 6 alkoxy.
5. The compound of claim 4 wherein L represents a nucleophilic group from the molecule.
6. The compound of claim 5 wherein the nucleophilic group comprises —NH, —S—, —O—, or —OOC—.