Microreactor
The present invention provides a reactive microreactor wherein a group of the following formula (1) which can bind to a member of specifically binding partner, is bound to a part or entirety of a wall surface of a channel: -L-SO 2 —X 1 (1) wherein X 1 represents —CR 1 ═CR 2 R 3 or —CHR 1 —CR 2 R 3 Y; R 1 , R 2 and R 3 independently represent an atom or a group selected from the group consisting of a hydrogen atom, a C1-6 alkyl group, a C6-20 aryl group, and a C7-20 aralkyl group having a C1-6 alkyl chain; Y represents a group which can be substitutable by a nucleophilic reagent or a group which is released as “HY” by a base; and L represents a linking group. The microreactor according to the present invention has advantages that it requires a less amount of a reagent and achieves a higher S/N ratio.
1 . (canceled)
2 . A method for producing a reactive microreactor wherein a group of the following formula (1) which can bind to a member of specifically binding partner, is bound to a part or entirety of a wall surface of a channel:
-L-SO 2 —X 1 (1)
wherein X 1 represents —CR 1 ═CR 2 R 3 or —CHR 1 —CR 2 R 3 Y; R 1 , R 2 and R 3 independently represent an atom or a group selected from the group consisting of a hydrogen atom, a C1-6 alkyl group a C6-20 aryl group and a C7-20 aralkyl group having a C1-6 alkyl chain; Y represents a group which can be substitutable by a nucleophilic reagent or a group which is released as “HY” by a base: and L represents a linking group;
the method comprising contacting a microreactor where a reactive group is introduced on the surface with a disulfon compound of the following formula (2):
X 1 —SO 2 -L 2 -SO 2 —X 2 (2)
wherein X 1 and X 2 independently represent —CR 1 ═CR 2 R 3 or —CHR 1 —CR 2 R 3 Y; R 1 , R 2 and R 3 independently represent an atom or a group selected from the group consisting of a hydrogen atom, a C1-6 alkyl group, a C6-20 aryl group and a C7-26 aralkyl group having a C1-6 alkyl chain; Y represents a group which can be substituted by a nucleophilic reagent or a group which is released as “HY” by a base; and L 2 represents a linking group.
3 - 15 . (canceled)
16 . A method for producing a biological material-bound microreactor wherein a group of the following formula (3) having a residual group of a member of specifically binding partner is bound to a part or entirety of a wall surface of a channel
-L-SO 2 —X-A (3)
wherein L represents a linking group which binds —SO 2 —X-A to the wall surface of the channel inside the microreactor; X represents —CR 11 (R 12 )—CR 13 (R 14 )—; R 11 , R 12 , R 13 and R 14 independently represent a hydrogen atom, a C1-6 alkyl group, a C6-20 aryl group or a C7-26 aralkyl group having a C1-6 alkyl chain; A represents a residual group of the member of the specifically binding partner;
the method comprising a step of contacting a reactive microreactor of wherein a group of the following formula (1) which can bind to a member of specifically binding partner, is bound to a part or entirety of a wall surface of a channel:
-L-SO 2 —X 1 (1)
wherein X 1 represents —CR 1 ═CR 2 R 3 or —CHR 1 —CR 2 R 3 Y R 1 , R 2 and R 3 independently represent an atom or a group selected from the group consisting of a hydrogen atom, a C1-6 alkyl group, a C6-20 aryl group and a C7-20 aralkyl group having a C1-6 alkyl chain; Y represents a group which can be substitutable by a nucleophilic reagent or a group which is released as “HY” by a base; and L represents a linking group, with at least one of a member of specifically binding partner having a reactive group which reacts with a group of the aforementioned formula (1) and forms a covalent bond.
17 . The method according to claim 16 , wherein the wall surface of the channel inside the microreactor is contacted with at least one of a member of specifically binding partner, and then a free reactive group present on the surface is subjected to blocking treatment with an aqueous solution of an amino acid, a peptide or a protein.
18 . (canceled)