A Canis sphingosine-1-phosphate (S1P) receptor isoform 1 (cS1P 1 ), the nucleic acid encoding the cS1P 1 receptor, and methods for using the cS1P 1 receptor and the nucleic acid encoding the cS1P 1 receptor in assays for identifying analytes which modulate activity of the cS1P 1 receptor.
1. An isolated nucleic acid molecule comprising a nucleotide sequence encoding a Canis sphingosine-1-phosphate isoform 1 (cS1P 1 ) receptor which comprises the amino acid sequence set forth in SEQ ID NO:2.
2. An isolated nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:1.
3. An isolated protein comprising the amino acid sequence Of SEQ ID NO:2.
4. A method for identifying an analyte that modulates activity of a Canis sphingosine-1-phosphate receptor isoform 1 (cS1P 1 ) receptor, which comprises:
(a) providing a recombinant cell comprising an isolated nucleic acid molecule which encodes a cS1P 1 receptor comprising the amino acid sequence set forth in SEQ ID NO: 2;
(b) incubating the recombinant cell in a medium with the analyte such that the cell expresses the cS1P 1 receptor; and
(c) determining the activity of the cS1P 1 receptor wherein a change in the activity of the cS1P 1 receptor indicates the analyte modulates activity of the cS1P 1 receptor.
5. The method of claim 4 wherein the activity of the cS1P 1 is determined by measuring a change in the intracellular concentration of Ca 2+ in the presence of the analyte.
6. The method of claim 4 wherein the activity of the cS1P 1 is determined by measuring a change in the intracellular concentration of a metabolite selected from the group consisting of inositol triphosphate (IP 3 ) and diacylglycerol (DAG) in the presence of the analyte.
7. The method of claim 4 wherein the activity of the cS1P 1 is determined by measuring a change in the activity of phospholipase C beta (PLC β ) or protein kinase C (PKC) in the presence of the analyte.
8. The method of claim 4 wherein the activity of the cS1P 1 is determined by measuring a change in the synthesis of cyclic AMP (cAMP) in the presence of the analyte.
9. The method of claim 4 wherein the isolated nucleic acid molecule which encodes the cS1P 1 receptor comprises the nucleotide sequence set forth in SEQ ID NO:1.