IP Library › Granted Patent US 7,935,509
Granted Patent B2
US 7,935,509 · App. 11/660,110 · Granted May 3, 2011

Intracellular production of a nuclease

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Quick Facts
Patent No.
US 7,935,509
App. No.
11/660,110
Granted
May 3, 2011
Kind
B2
Abstract

Methods and compositions are provided that relate to obtaining a recombinant DNA and RNA cleaving nuclease. This involves the over-expression of a fusion protein between maltose-binding protein and a truncated nuclease in a soluble form in the cytoplasm of a host cell from which it can be readily extracted.

Claims (4)

1. An MBP-nuclease fusion protein comprising SEQ ID NO: 2 or SEQ ID NO: 4.

2. A method for preparing a non-viscous extract of a prokaryotic cell, comprising:

(a) adding an MBP-nuclease fusion protein comprising SEQ ID NO: 2 or SEQ ID NO: 4, decanoyl-N-methylglucamide and a lysozyme to a cell pellet or cell suspension; and

(b) preparing the non-viscous extract from the prokaryotic cells.

Assignments (3)
NOTICE OF GRANT OF SECURITY INTEREST IN PATENTS Recorded Sep 27, 2023
From: NEW ENGLAND BIOLABS, INC.
To: BANK OF AMERICA, N.A., AS ADMINISTRATIVE AGENT
Reel/Frame 065044/0729 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 12, 2007
From: SEEFELDT, ALAN JEFFREY; VINTON, MARK STUART
To: DOLBY LABORATORIES LICENSING CORPORATION
Reel/Frame 019242/0232 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 12, 2007
From: HSIEH, PEI-CHUNG
To: NEW ENGLAND BIOLABS, INC.
Reel/Frame 018933/0203 →