Antibiotic Compound
Fermentation of a nutrient medium with a Nocardia spp. yields a novel broad spectrum antibiotic compound of structural formula (I) or a pharmaceutically acceptable salt, ester, enantiomer, diasteriomer or mixture.
1 . A compound of structural formula I:
or a pharmaceutically acceptable salt, ester, enantiomer, diasteriomer or mixture thereof,
wherein:
R represents —C(O)NH 2 ; —C(O)NHC═CH2C(O)NH 2 ;
R 1 , R 2 , and R 3 independently represent OH;
or R 1 , R 2 and R 3 taken together represent:
R 4 represents OH,
and R 5 represents hydrogen or OH.
2 . A compound which is:
or pharmaceutically acceptable salts, esters, enantiomers, diastereomers or a mixture thereof.
3 . A process for the preparation of the compound of structural formula I of claim 1 , which comprises the cultivation of a Nocardia spp. with ATCC accession number 202099 or a natural or artificial mutant thereof in a nutrient medium and recovering the compound of structural formula I from the fermentation broth.
4 . The process of claim 3 wherein the fermentation is conducted at a temperature of about 10° C. to about 40° C.
5 . The process of claim 4 , wherein the fermentation is conducted at a temperature of about 31-32° C.
6 . A pharmaceutical composition comprising a pharmaceutically acceptable carrier and an effective amount of a compound of structural formula I of claim 1 .
7 . Use of a compound of formula I of claim 1 for the manufacture of a medicament for treating a bacterial infection in a host in need of such treatment.