Meningococcal outer membrane vesicles
View Patent ↗OMVs targeted against specific epidemic strains can be highly effective in controlling localized outbreaks of disease. In combination with large-scale and reproducible manufacturing techniques, a vaccine can be rapidly produced after an outbreak. The invention provides a method for preparing a meningococcal outer membrane vesicle (OMV) vaccine, comprising the steps of: (i) identifying the serosubtype of a meningococcal strain associated with an outbreak of meningococcal meningitis; (ii) preparing OMVs from a meningococcal strain having the serosubtype identified in step (i) for use in vaccine manufacture. The method may comprise one or both of the further steps of (iii) formulating said OMVs as a vaccine; and (iv) distributing said vaccine in a geographical area affected by or likely to be affected by said outbreak. The meningococcal strain will typically be in serogroup B, but may be instead by in serogroup A, C, W135, Y, etc.
1. A composition comprising an admixture of outer membrane vesicles (OMVs) from a first meningococcus strain and a second meningococcus strain, wherein the first meningococcus strain is serosubtype P1.7-2,4, the concentration of OMVs from the first meningococcus strain is about 25 μg protein/mL, the concentration of OMVs from the second meningococcus strain is about 25 μg protein/mL, and the volume of the composition is 0.5 mL.
2. The composition of claim 1 , further comprising a conjugated capsular saccharide from one or more of meningococcal serogroups A, C, W135 and Y.
3. The composition of claim 1 , wherein the first or the second meningococcus strain has a knockout of one or more enzymes involved in LPS biosynthesis.
4. The composition of claim 1 , wherein the first or the second meningococcus strain over-expresses an immunogen selected from the group consisting of NspA, protein 287, protein 741, TbpA, TbpB, and superoxide dismutase.
5. The composition of claim 1 , comprising less than 0.4 μg of deoxycholate for every μg of protein.
6. The composition of claim 1 , comprising less than 0.12 μg of meningococcal LPS for every μg of protein.
7. The composition of claim 1 , comprising an aluminum hydroxide adjuvant.
8. The composition of claim 1 , wherein the composition includes an aluminum hydroxide adjuvant and a histidine buffer.
9. The composition of claim 1 , further comprising a conjugated capsular saccharide from two or more of meningococcal serogroups A, C, W135 and Y.
10. A kit comprising separate containers of outer membrane vesicles (OMVs) from a first meningococcus strain and a second meningococcus strain, wherein the first meningococcus strain is serosubtype P1.7-2,4 and after resuspension of the OMVs from both containers in 0.5 mL, the concentration of OMVs from the first meningococcus strain is about 25 μg protein/mL and the concentration of OMVs from the second meningococcus strain is about 25 μg protein/mL.
11. The kit of claim 10 , further comprising a conjugated capsular saccharide from one or more of meningococcal serogroups A, C, W135 and Y.
12. A method for administering a meningococcal OMV vaccine comprising the composition of claim 1 to a patient, wherein a first dose is given at time zero, a second and a third dose are given over the next two months, and a fourth dose is given between 11 and 13 months after time zero.