Method of culturing a microorganism under controlled agitation and aeration conditions
View Patent ↗A method of culturing a microorganism capable of producing at least any one of a highly unsaturated fatty acid or a compound containing a highly unsaturated fatty acid as a constituent fatty acid in a culture medium containing at least a carbon source and a nitrogen source, using an aeration-agitation culture device capable of adjusting and controlling an agitation power and an aeration amount. The method comprises the steps of performing mechanical agitation for a predetermined time after start of culture, where the agitation power per unit liquid amount is 269 (W/m 3 ) or less, and after the predetermined time has passed, adjusting and controlling at least any one of the maximum aeration amount and a maximum power required for agitation to a range which satisfies that KLA (=(P/V) 0.95 Vs 0.67 ) is 59 or more, an air flow rate parameter Vs 0.67 is 0.075 or more, and a required agitation power parameter (P/V) 0.95 is 203 or more, where P represents power required for agitation (W), V represents a liquid amount (m 3 ), and Vs represents an air flow rate (m/sec).
1. A method of producing a highly unsaturated fatty acid by culturing a strain of Mortierella alpina capable of producing the highly unsaturated fatty acid in a culture medium containing at least a carbon source and a nitrogen source, using an aeration-agitation culture device capable of adjusting and controlling an agitation power and an aeration amount, the method comprising:
performing mechanical agitation after start of a main culture, wherein the agitation power per unit liquid amount is 30-269 (W/m 3 )thereby promoting a transformation of M. alpina cells from a form of a pulp-shaped hypha to a pellet-shaped form, and wherein the main culture is started by inoculating a seed culture; and
immediately before dissolved oxygen concentration value decreases to about 50% or 12 to 24 hours after start of the main culture, increasing aeration or agitation by adjusting and controlling at least any one of the maximum aeration amount and a maximum power required for agitation to a range which satisfies that KLA (=(P/V) 0.95 Vs 0.67 ) is 59-282, an air flow rate parameter Vs 0.67 is 0.075-0.191, and a required agitation power parameter (P/V) 0.95 is 282-2169, where P represents power required for agitation (W), V represents a liquid amount (m 3 ), and Vs represents an air flow rate (m/sec),
thereby producing the highly unsaturated fatty acid from the main culture.
2. The method according to claim 1 , wherein the highly unsaturated fatty acid is arachidonic acid.
3. A method of producing a compound containing a highly unsaturated fatty acid by culturing a strain of Mortierella alpina capable of producing the compound containing the highly unsaturated fatty acid in a culture medium containing at least a carbon source and a nitrogen source, using an aeration-agitation culture device capable of adjusting and controlling an agitation power and an aeration amount, the method comprising:
performing mechanical agitation after start of a main culture, wherein the agitation power per unit liquid amount is 30-269 (W/m 3 ) thereby promoting a transformation of M. alpine cells from a form of pulp-shaped hypha to a pellet-shaped form, and wherein the main culture is started by inoculating a seed culture; and
immediately before dissolved oxygen concentration value decreases to about 50% or 12 to 24 hours after start of the main culture, increasing aeration or agitation by adjusting and controlling at least any one of the maximum aeration amount and a maximum power required for agitation to a range which satisfies that KLA (=(P/V) 0.95 Vs 0.67 ) is 59-282, an air flow rate parameter Vs 0.67 is 0.075-0.191, and a required agitation power parameter (P/V) 0.95 is 282-2169, where P represents power required thr agitation (W), V represents a liquid amount (m 3 ), and Vs represents an air flow rate (m/sec),
thereby producing the compound containing the highly unsaturated fatty acid from the main culture.
4. The method according to claim 3 , wherein the highly unsaturated fatty acid is arachidonic acid.
5. The method according to claim 1 , wherein (P/V) 0.95 is in the range of 282-2169 and Vs 0.67 is in the range of 0.075-0.191 after increasing aeration or agitation.
6. The method according to claim 1 , wherein (P/V) 0.95 is in the range of 282-2169 and KLA (=(P/V) 0.95 Vs 0.67 ) is in the range of 59-282 after increasing aeration or agitation.
7. The method according to claim 3 , wherein (P/V) 0.95 is in the range of 282-2169 and Vs 0.67 is in the range of 0.075-0.191 after increasing aeration or agitation.
8. The method according to claim 3 , wherein (P/V) 0.95 is in the range of 282-2169 and KLA (=(P/V) 0.95 Vs 0.67 ) is in the range of 59-282 after increasing aeration or agitation.