Diagnostic agent for tumor
View Patent ↗According to the present invention, a tumor diagnostic agent which carries out early stage detection, monitoring of therapeutic effect and prognostic diagnosis of all tumors The tumor diagnostic agent of the present invention is a tumor diagnostic agent comprising δ-aminolevulinic acid represented by formula (I), a derivative thereof and a salt thereof: R 2 R 1 NCH 2 COCH 2 CH 2 COR 3 (I) wherein R 1 and R 2 each independently represents hydrogen, alkyl, acyl, alkoxycarbonyl, aryl or aralkyl; and R 3 represents hydroxy, alkoxy, acyloxy, alkoxycarbonyloxy, aryloxy, aralkyloxy or amino, which is used for diagnoses by measuring porphyrins in a sample collected from the inside or outside of the body after its administration.
1. A method for detecting the presence of tumors which comprises orally administering to a subject in need of said detecting δ-aminolevulinic acid represented by formula (I):
R 2 R 1 NCH 2 COCH 2 CH 2 COR 3 (I)
wherein R 1 and R 2 each independently represents hydrogen and R 3 represents hydroxy or alkoxy or a salt thereof, collecting a sample from said subject and then measuring the concentration of porphyrins in the sample to thereby detect the presence of said tumors,
wherein the sample is collected from the inside or outside of the body and is blood, body fluid, urine, feces, saliva, sweat, cerebrospinal fluid, semen or tears,
wherein the porphryins are uroporphyrin, coproporphryin, uroporphyrin I, uroporphyrin III, coproporphyrin I, or coproporphyrin III, and
wherein the sample is collected from the inside or outside of the body after from 0.1 to 10 hours of the administration of the δ-aminolevulinic acid or salt thereof.
2. The method according to claim 1 , wherein the tumor is in the brain, nasal tract, nasal cavity, trachea, bronchus, oral cavity, pharynx, esophagus, stomach, breast, colorectum, lung, ovary, central nervous system, liver, bladder, urethra, ureter, pancreas, cervical duct, abdominal cavity, anal canal or cervix uteri.
3. The method according to claim 1 , wherein uroporphyrin I and uroporphyrin III are measured, and a tumor is detected based on the uroporphyrin/uroporphyrin III concentration ratio.
4. The method according to claim 1 , wherein the porphyrins are measured using HPLC (high performance liquid chromatography), TLC (thin layer chromatography), fluorescence detection or immunoassay.
5. The method according to claim 1 , wherein the porphyrins are measured using HPLC (high performance liquid chromatography) or TLC (thin layer chromatography).