Methods for the preparation, isolation and purification of epothilone B, and x-ray crystal structures of epothilone B
View Patent ↗The present invention relates to improved methods for the production, isolation and purification of epothilone B. These methods include, for example, a fermentation process for the production of epothilone B, isolation via adsorption onto a resin, and subsequent purification.
1. A strain of Sorangium cellulosum deposited as ATCC No. PTA-3880 or a strain of Sorangium cellulosum deposited as ATCC No. PTA-3881.
2. The strain of claim 1 deposited as ATCC No. PTA-3880.
3. The strain of claim 1 deposited as ATCC No. PTA-3881.
4. The strain of claim 1 , wherein said strain is capable of producing epothilone B in a recoverable amount upon fermentation in a nutrient medium.
5. The strain of claim 4 , wherein said strain is capable of improving the production ratio of epothilone B to epothilone A.
6. The strain of claim 5 , wherein said ratio of epothilone B to epothilone A ratio is at least 1.
7. The strain of claim 6 , wherein said ratio of epothilone B to epothilone A ratio is at least 1.5.
8. The strain of claim 7 , wherein said ratio of epothilone B to epothilone A ratio is in the range of 1.5 to 4.0.