DRY POWDER CELL CULTURE PRODUCTS AND METHODS OF PRODUCTION THEREOF
The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.
1 . A method of producing a nutritive medium powder, the method comprising agglomerating a dry powder nutritive medium with a solvent.
2 . A dry powder animal cell culture medium with a bulk density between from about 0.5449 g/ml to about 0.6461 g/ml.
3 . The dry powder animal cell culture medium of claim 2 , wherein the bulk density is selected from the group consisting of between from about 0.5669 g/ml to about 0.6048 g/ml, from about 0.5449 g/ml to about 0.6148 g/ml and from about 0.5856 g/ml to about 0.6341 g/ml.
4 . The dry powder animal cell culture medium of claim 2 , wherein the bulk density is selected from the group consisting of a bulk density between from about 0.5784 g/ml to about 0.6461 g/ml, from about 0.5928 g/ml to about 0.5726 g/ml, and from about 0.5475 g/ml to about 0.5953 g/ml.
5 . The dry powder animal cell culture medium of claim 2 , wherein the dry powder animal cell culture medium is suitable for culturing an animal cell selected from the group consisting of an insect cell, a nematode cell, a human cell and a mammalian cell.
6 . The dry powder animal cell culture medium of claim 2 , wherein the dry powder animal cell culture medium is suitable for culturing a cell selected from the group consisting of an embryonic cell, a Drosophila cell, a Spodoptera cell, a Trichoplusa cell, a C. elegans cell, a CHO cell, a COS cell, a VERO cell, a BHK cell, an AE-1 cell, a SP2/0 cell and a L5.1 cell.
7 . The dry powder animal cell culture medium of claim 2 , comprising one or more ingredients selected from the group consisting of L-glutamine, insulin, transferrin, a lipid, a cytokine, a neurotransmitter and a buffer.
8 . (canceled)
9 . (canceled)
10 . (canceled)
11 . (canceled)
12 . The dry powder animal cell culture medium of claim 7 , wherein the buffer is sodium bicarbonate.
13 . The dry powder animal cell culture medium of claim 2 , wherein upon reconstitution with water the reconstituted culture medium, is at the desired pH for culturing an animal cell.
14 . The dry powder animal cell culture medium of claim 2 , wherein the dry powder has been agglomerated.
15 . The dry powder animal cell culture medium of claim 7 , wherein the lipid is selected from the group consisting of:
(a) a phospholipid;
(b) a sphingolipid;
(c) a fatty acid; and
(d) a cholesterol
16 . A nutritive medium powder prepared by agglomerating a dry powder nutritive medium with a solvent.
17 . The nutritive medium powder of claim 16 , wherein the dry powder nutritive medium is prepared by spray-drying a liquid nutritive medium to form a powder.
18 . The nutritive medium powder of claim 16 , wherein the solvent is an aqueous solvent.
19 . A fluid bed apparatus for producing granular materials from powders, the
fluid bed apparatus comprising:
(a) an inlet for injecting a solvent; and
(b) a dry powder cell culture medium component.
20 . The fluid bed apparatus of claim 19 , wherein the dry powder cell culture medium component is a component selected from the group consisting of:
(a) a culture medium;
(b) a culture medium supplement; and
(c) a culture medium subgroup.