Parthenolide free bioactive ingredients from feverfew (
View Patent ↗The present invention relates to bioactive ingredients that include isolated bioactive fractions derived from cell juice of fresh biomass of a feverfew ( Tanacetum parthenium ) plant. The bioactive fractions are either free of or substantially free of α-unsaturated γ-lactones (e.g., parthenolide). Further, the bioactive fractions have anti-inflammatory and antioxidant activity. The present invention also relates to a method for isolating a bioactive fraction that is derived from cell juice of fresh biomass of a feverfew ( Tanacetum parthenium ) plant and that is at least substantially free of α-unsaturated γ-lactones (e.g., parthenolide). The present invention also relates to a method for preparing a stabilized cell juice serum fraction and a stabilized concentrated cell juice serum fraction that are free of α-unsaturated γ-lactones (e.g., parthenolide). The present invention also relates to a bioactive composition that includes a mixture of one or more of the isolated bioactive fractions of the present invention.
1. A method for isolating a bioactive fraction that is derived from cell juice of fresh biomass of a feverfew ( Tanacetum parthenium ) plant and that is substantially free of α-unsaturated γ-lactones, said method comprising:
providing fresh biomass of a feverfew ( Tanacetum parthenium ) plant;
processing the fresh biomass under conditions effective to yield a cell juice supernatant that is at least substantially free of α-unsaturated γ-lactones and a membrane fraction containing α-unsaturated γ-lactones, said processing the fresh biomass comprising:
(i) separating the fresh biomass into a cell juice component and a fiber enriched material; and
(ii) subjecting the cell juice component to a combined pH adjustment and microwave treatment step to yield the cell juice supernatant that is at least substantially free of α-unsaturated γ-lactones, wherein the pH adjustment comprises decreasing the pH to a range of 3 to 4;
treating the cell juice supernatant under conditions effective to yield a first cell juice serum supernatant and a cytoplasm fraction precipitate; and
isolating the cytoplasm fraction precipitate, wherein said cytoplasm fraction precipitate is a bioactive fraction that is substantially free of α-unsaturated γ-lactones, wherein said α-unsaturated γ-lactones comprise parthenolide.
2. The method according to claim 1 , wherein the step of processing the fresh biomass further comprises:
clarifying the cell juice component to yield a clarified cell juice component prior to subjecting the cell juice component to the combined pH adjustment and microwave treatment step.
3. The method according to claim 1 further comprising:
clarifying the first cell juice serum supernatant under conditions effective to yield a second cell juice serum supernatant;
mixing a stabilizing agent with the second cell juice serum supernatant under conditions effective to yield a stabilized cell juice serum fraction,
wherein said stabilized cell juice serum fraction is a bioactive fraction that is substantially free of α-unsaturated γ-lactones, said α-unsaturated γ-lactones comprising parthenolide.
4. The method according to claim 3 further comprising:
concentrating the stabilized cell juice serum fraction under conditions effective to yield a concentrated cell juice serum supernatant.
5. The method according to claim 4 further comprising:
mixing a stabilizing agent with the concentrated cell juice serum supernatant under conditions effective to yield a stabilized concentrated cell juice serum fraction,
wherein said stabilized concentrated cell juice serum fraction is a bioactive fraction that is free of α-unsaturated γ-lactones, said α-unsaturated γ-lactones comprising parthenolide.