Rapid growing microorganisms for biotechnology applications
View Patent ↗The present invention provides novel rapidly growing microorganisms and methods for their use in cloning or subcloning nucleic acid molecules. The rapid growing microorganisms of the present invention form colonies more rapidly than microorganisms typically used in molecular biology and thus provide a significant improvement in in vitro cloning methods used extensively in molecular biology. The invention also relates to kits and compositions used in the methods of the invention.
1. A method of transformation comprising:
(a) transforming competent E. coli with at least one vector;
(b) incubating transformed E. coli containing said at least one vector on an appropriate growth medium; and
(c) observing visible colonies comprising said transformed E. coli 7.8 to 8.5 hours after said transformation and incubation,
wherein the growth characteristics of said competent E. coli are such that the E. coli exhibits visible colonies 7.8 to 8.5 hours after transformation and incubation on LB agar at 37° C.
2. The method according to claim 1 , wherein said E. coli is lacZ − .
3. The method according to claim 2 , wherein said E. coli is lacZΔM15.
4. The method according to claim 1 , wherein said E. coli is recA − .
5. The method according to claim 1 , wherein said E. coli is restriction negative.
6. The method according to claim 1 , wherein said E. coli is modification negative.
7. The method according to claim 1 , wherein said E. coli is restriction negative and modification negative.
8. The method according to claim 1 , wherein said E. coli is methylase deficient.
9. The method according to claim 8 , wherein said E. coli is mcrA − .
10. The method according to claim 8 , wherein said E. coli is mcrB − .
11. The method according to claim 1 , wherein said E. coli is endonuclease deficient.
12. The method according to claim 11 , wherein said E. coli is endA1.
13. The method according to claim 1 , wherein the E. coli is suitable for alpha-complementation and selection by blue/white screening.