IP Library › Patent Application 11680259
Patent Application
App. No. 11/680,259

NOVGEL METHODS FOR DISPLAYING (POLY)PEPTIDES/PROTEINS ON BACTERIOPHAGE PARTICLES VIA DISULFIDE BONDS

Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US None
App. No.
11/680,259
Abstract

The present invention relates to methods for displaying (poly)peptides/proteins on the surface of bacteriophage particles by attaching the (poly)peptide/proteins via disulfide bonds.

Claims (55)

1 - 31 . (canceled)

32 . An isolated host cell comprising:

(a) a first vector comprising a nucleic acid sequence encoding a variant of a wild type coat protein of a bacteriophage, wherein said variant comprises:

(aa) one or more parts of said wild type coat protein of a bacteriophage, wherein one of said parts comprises at least that part which causes or allows the incorporation of said coat protein into the phage coat; and,

(ab) between one and six additional amino acid residues not present at the corresponding amino acid positions in a wild type coat protein of a bacteriophage, wherein one of said additional amino acid residues is a cysteine residue, and

(b) a second vector comprising one or more nucleic acid sequences encoding a (poly)peptide/protein comprising a cysteine residue.

33 . The isolated host cell of claim 32 , wherein said bacteriophage is a filamentous bacteriophage.

34 . An isolated host cell comprising:

(a) a first vector comprising a nucleic acid sequence encoding a wild type coat protein or a truncated portion thereof of a bacteriophage, wherein said wild type coat protein or said truncated portion thereof comprises:

(aa) one or more parts of said wild type coat protein or said truncated variant thereof of a bacteriophage, wherein one of said parts comprises at least that part which causes or allows the incorporation of said coat protein into the phage coat; and,

(ab) a cysteine residue which is present at a corresponding amino acid position in a wild type coat protein or a truncated portion thereof of a bacteriophage, and

(b) a second vector comprising one or more nucleic acid sequences encoding a (poly)peptide/protein comprising a cysteine residue.

35 . The isolated host cell of claim 34 , wherein said bacteriophage is a filamentous bacteriophage.

36 . The isolated host cell of claim 32 , wherein said nucleic acid sequence encoding said variant of said wild type coat protein further encodes:

(c) one or more peptide sequences for purification and/or detection purposes, wherein said one or more peptide sequences are fused to said variant of said wild type coat protein.

37 . The isolated host cell of claim 34 , wherein said nucleic acid sequence encoding said wild type coat protein or said truncated portion thereof further encodes:

(c) one or more peptide sequences for purification and/or detection purposes, wherein said one or more peptide sequences are fused to said variant of said wild type coat protein.

38 . The isolated host cell of claim 32 , wherein said host cell is a bacterial, fungal, plant, insect or mammalian host cell.

39 . The isolated host cell of claim 34 , wherein said host cell is a bacterial, fungal, plant, insect or mammalian host cell.

40 . The isolated host cell of claim 32 , wherein said (poly)peptide/protein comprises an immunoglobulin or a functional fragment thereof.

41 . The isolated host cell of claim 34 , wherein said (poly)peptide/protein comprises an immunoglobulin or a functional fragment thereof.

42 . A vector comprising

(a) a nucleic acid sequence encoding a variant of a wild type coat protein of a bacteriophage, wherein said variant comprises:

(aa) one or more parts of said wild type coat protein of a bacteriophage, wherein one of said parts comprises at least that part which causes or allows the incorporation of said coat protein into the phage coat; and

(ab) between one and six additional amino acid residues not present at the corresponding amino acid positions in a wild type coat protein of a bacteriophage, wherein one of said additional amino acid residues is a cysteine residue; and

(b) one or more nucleic acid sequences encoding a (poly)peptide/protein comprising a cysteine residue.

43 . The vector of claim 42 , wherein said bacteriophage is a filamentous bacteriophage.

44 . The vector of claim 42 , wherein the expression product of said nucleic acid sequence encoding said variant of a wild type coat protein and the expression product of said nucleic acid sequences encoding a (poly)peptide/protein do not form a genetic fusion protein.

45 . The vector of claim 42 , wherein no interaction domain for interaction with a second domain present in the (poly)peptide/protein has been recombinantly fused to said coat protein.

46 . The vector of claim 42 , wherein said (poly)peptide/protein comprises an immunoglobulin or a functional fragment thereof.

47 . The vector of claim 44 , wherein said (poly)peptide/protein comprises an immunoglobulin or a functional fragment thereof.

48 . The vector of claim 45 , wherein said (poly)peptide/protein comprises an immunoglobulin or a functional fragment thereof.

49 . An isolated host cell comprising a vector of claim 42 .

50 . An isolated host cell comprising a vector of claim 43 .

51 . An isolated host cell comprising a vector of claim 44 .

52 . An isolated host cell comprising a vector of claim 45 .

53 . An isolated host cell comprising a vector of claim 46 .

54 . An isolated host cell comprising a vector of claim 47 .

55 . An isolated host cell comprising a vector of claim 48 .

56 . The isolated host cell of claim 49 , wherein said host cell is a bacterial, fungal, plant, insect or mammalian host cell.

57 . The isolated host cell of claim 50 , wherein said host cell is a bacterial, fungal, plant, insect or mammalian host cell.

58 . The isolated host cell of claim 51 , wherein said host cell is a bacterial, fungal, plant, insect or mammalian host cell.

59 . The isolated host cell of claim 52 , wherein said host cell is a bacterial, fungal, plant, insect or mammalian host cell.

60 . The isolated host cell of claim 53 , wherein said host cell is a bacterial, fungal, plant, insect or mammalian host cell.

61 . The isolated host cell of claim 54 , wherein said host cell is a bacterial, fungal, plant, insect or mammalian host cell.

62 . The isolated host cell of claim 55 , wherein said host cell is a bacterial, fungal, plant, insect or mammalian host cell.

63 . The isolated host cell of claim 49 , wherein said bacteriophage is a filamentous bacteriophage.

64 . An isolated nucleic acid sequence encoding

(a) a variant of a wild type coat protein of a bacteriophage, wherein said variant comprises:

(aa) one or more parts of said wild type coat protein of a bacteriophage, wherein one of said parts comprises at least that part which causes or allows the incorporation of said coat protein into the phage coat; and

(ab) between one and six additional amino acid residues not present at the corresponding amino acid positions in a wild type coat protein of a bacteriophage, wherein one of said additional amino acid residues is a cysteine residue; and

(b) a (poly)peptide/protein comprising a cysteine residue.

65 . The isolated nucleic acid of claim 64 , wherein said (poly)peptide/protein comprising the cysteine residue is selected from the group consisting of immunoglobulins, VH, VL, Fv, scFv, disulfide-linked Fv, Fab and F(ab′)2.

66 . An isolated host cell comprising the nucleic acid sequence of claim 64 .

67 . An isolated host cell comprising the nucleic acid sequence of claim 65.