Highly phosphorylated and sulfated recombinant factor IX
View Patent ↗The present invention relates to a purified recombinant blood coagulation factor IX (rFIX) preparation, wherein at least 25% of the rFIX in the preparation is fully phosphorylated and sulfated, a cell culture expressing a rFIX resulting in said preparation, a pharmaceutical composition for treating a bleeding disorder comprising said preparation, and a method for treating a bleeding disorder comprising the step of administering said preparation to a patient in need thereof.
1. A preparation comprising purified human recombinant blood coagulation factor IX (rFIX), wherein at least 25% of the rFIX in the preparation is fully phosphorylated and sulfated.
2. A pharmaceutical composition for treating a bleeding disorder, the composition comprising a preparation according to claim 1 and a pharmaceutically acceptable carrier.
3. The pharmaceutical composition according to claim 2 , wherein the bleeding disorder is hemophilia B.
4. A method of treating a bleeding disorder, the method comprising the step of administering an effective amount of a pharmaceutical composition according to claim 2 to a patient in need thereof.
5. The method of claim 4 , wherein the patient has been diagnosed with Hemophilia B.
6. The preparation of claim 1 wherein less than 98% of the rFIX in the preparation is fully phosphorylated and sulfated.