Method to detect IgE
View Patent ↗The present invention includes a method to detect IgE using a human Fc epsilon receptor (Fc 68 R) to detect IgE antibodies in a biological sample from a cat, a dog, or a horse. The present invention also relates to kits to perform such methods.
1. A method to detect a plant allergy in a canid, felid or equid comprising:
(a) contacting an allergen from a plant with a putative IgE containing composition from said animal, under conditions suitable for formation of an allergen:IgE complex;
(b) contacting the putative allergen:IgE complex with a human FcεR molecule under conditions suitable for formation of an allergen:IgE:FcεR molecule complex, wherein either the allergen or the human FcεR molecule is labeled by conjugation to a detectable marker;
(c) washing the allergen:IgE:FcεR mixture to remove non-specifically bound labeled- molecule; and
(d) detecting the presence of an allergen:IgE:FcεR complex, if present, by detecting the presence of detectable marker, wherein the presence of an allergen:IgE:FcεR complex indicates the animal is allergic to said plant.
2. The method of claim 1 , wherein said putative IgE containing composition comprises an animal fluid is selected from the group consisting of serum, plasma, blood, urine, tears, saliva, nasal secretions and milk.
3. The method of claim 1 , wherein said allergen is selected from the group consisting of a grass allergen, a plantain allergen, a pigweed allergen, a ragweed allergen, a sage allergen, an elm allergen, a cocklebur allergen, an elder allergen, a walnut allergen, a cottonwood allergen, an ash allergen, a birch allergen, a cedar allergen, an oak allergen, and a mulberry allergen.
4. The method of claim 1 , wherein said allergen is selected from the group consisting of a Johnson Grass allergen, a Kentucky Blue Grass allergen, a Meadow Fescue allergen, an Orchard Grass allergen, a Perennial Rye Grass allergen, a Redtop Grass allergen, a Timothy Grass allergen, a Bermuda Grass allergen, a Brome Grass allergen, a Curly Dock allergen, an English Plantain allergen, a Mexican Firebush allergen, a Lamb's Quarters allergen, a Rough Pigweed allergen, a Short Ragweed allergen, a Wormwood Sage allergen, an American Elm allergen, a Common Cocldebur allergen, a Box Elder allergen, a Black Walnut allergen, an Eastern Cottonwood allergen, a Green Ash allergen, a River Birch allergen, a Red Cedar allergen, a Red Oak allergen, and a Red Mulberry allergen.
5. The method of claim 1 , wherein said FcεR molecule is encoded by a nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:4, SEQ ID NO:7, SEQ ID NO:10 and SEQ ID NO:12.
6. The method of claim 1 , wherein said allergen is bound to a substrate.
7. A method to detect plant-allergen-specific IgE in a canid, felid or equid, said method comprising:
(a) contacting a plant allergen with a putative IgE containing composition from said animal, under conditions suitable for formation of an allergen:IgE complex;
(b) contacting the putative allergen:IgE complex with a human FcεR molecule under conditions suitable for formation of an allergen:IgE:FcεR molecule complex, wherein either the allergen or the human FcεR molecule is labeled by conjugation to a detectable marker;
(c) washing the allergen:IgE:FcεR mixture to remove non-specifically bound lableled-molecule; and
(d) detecting the presence of an allergen:IgE:FcεR complex, if present, by detecting the presence of detectable marker, wherein the presence of an allergen:IgE:FcεR molecule complex indicates the presence of plant-allergen-specific IgE.
8. The method of claim 7 , wherein said putative IgE containing composition comprises an animal fluid is selected from the group consisting of serum, plasma, blood, urine, tears, saliva, nasal secretions and milk.
9. The method of claim 7 , wherein said allergen is selected from the group consisting of a grass allergen, a plantain allergen, a pigweed allergen, a ragweed allergen, a sage allergen, an elm allergen, a cocklebur allergen, an elder allergen, a walnut allergen, a cottonwood allergen, an ash allergen, a birch allergen, a cedar allergen, an oak allergen, and a mulberry allergen.
10. The method of claim 7 , wherein said allergen is selected from the group consisting of a Johnson Grass allergen, a Kentucky Blue Grass allergen, a Meadow Fescue allergen, an Orchard Grass allergen, a Perennial Rye Grass allergen, a Redtop Grass allergen, a Timothy Grass allergen, a Bermuda Grass allergen, a Brome Grass allergen, a Curly Dock allergen, an English Plantain allergen, a Mexican Firebush allergen, a Lamb's Quarters allergen, a Rough Pigweed allergen, a Short Ragweed allergen, a Wormwood Sage allergen, an American Elm allergen, a Common Cocklebur allergen, a Box Elder allergen, a Black Walnut allergen, an Eastern Cottonwood allergen, a Green Ash allergen, a River Birch allergen, a Red Cedar allergen, a Red Oak allergen, and a Red Mulberry allergen.
11. The method of claim 7 , wherein said FcεR molecule is encoded by a nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:4, SEQ ID NO:7, SEQ ID NO:10 and SEQ ID NO:12.
12. The method of claim 7 , wherein said allergen is bound to a substrate.