Reduction of platelet interference in plasma assay samples
View Patent ↗The present invention relates to compositions for improving assay accuracy for plasma samples by decreasing or eliminating false results due to platelet interference. The compositions of the present invention comprise compositions that include a platelet interference reducing agent in an amount effective to decrease the platelet interference activity in the plasma sample, particularly platelet-rich plasma sample, to be analyzed. The most preferred platelet interference reducing agent of the present invention is 1-(1,3-Bis(hydroxymethyl-2,5-dioxoimidazolidin-4-iyl)-1,3-bis(hydroxymethyl) urea, also called “Diazolidinyl urea” or “DZU”.
1. A method of reducing platelet interference in an assay, comprising: contacting a sample comprising platelets with a composition comprising diazolidinyl urea to inhibit platelet activation in the sample, thereby forming a combination; and, measuring at least one analyte present in the sample in said combination.
2. The method according to claim 1 wherein said measuring at least one analyte is performed using one or more immunoassays.
3. The method according to claim 2 wherein at least one of said one or more immunoassays is an assay that is performed within a short period of time following sample acquisition.
4. The method according to claim 1 wherein said at least one analyte is human choriongonadotropin.
5. The method according to claim 1 wherein said at least one analyte is a cardiac marker.
6. The method according to claim 1 wherein said combination comprises from about 1.5 weight percent to about 7 weight percent diazolidinyl urea, wherein weight percents are based upon the total weight of said combination.
7. The method according to claim 1 wherein said combination comprises from about 3 weight percent to about 7 weight percent diazolidinyl urea, wherein weight percents are based upon the total weight of said combination.
8. The method according to claim 1 wherein said combination comprises from about 3 weight percent to about 6 weight percent diazolidinyl urea, wherein weight percents are based upon the total weight of said combination.
9. The method according to claim 1 wherein said combination comprises from about 4.5 weight percent to about 5.5 weight percent diazolidinyl urea, wherein weight percents are based upon the total weight of said combination.