Vaccines
The present invention relates to compositions for inducing immune responses, including an antigen and a promiscuous T-cell epitope. Also provided are methods of inducing immune responses in hosts, comprising administering compositions comprising antigens and promiscuous T-cell epitopes to the host.
1. A composition comprising:
(a) a truncated VP2 antigen of infectious pancreatic necrosis virus (IPNV), wherein said truncated VP2 antigen is selected from the group consisting of SEQ ID NO: 146 SEQ ID NO: 148, SEQ ID NO: 150 and SEQ ID NO: 152; and
(b) optionally including a promiscuous T-cell epitope comprising a peptide having the amino acid sequence of selected from group consisting of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, or a combination thereof.
2. The composition of claim 1 , further comprising a pharmaceutically acceptable carrier.
3. The composition of claim 1 , further comprising an adjuvant.
4. The composition of claim 1 , wherein said composition further comprises a cytokine.
5. The composition of claim 4 , wherein said cytokine is selected from the group consisting of: interleukins (IL)-1 through -25, human B cell-activating factor (BAFF), granulocyte colony-stimulating factor (G-CSF), granulocyte/macrophage colony-stimulating factor (GM-CSF), interferon-alpha, interferon-beta, interferon-gamma, leukemia inhibitory factor (LIF), macrophage Colony stimulating factor (M-CSF), macrophage inhibition factor (MIF), oncostatin M (OSM), stem cell factor (CSF), thrombopoietin (Tpo), transforming growth factor beta (TGF-β) tumor necrosis factor-alpha (TNF-α) and tumor necrosis factor-beta (TNF-β).
6. The composition of claim 4 , wherein the cytokine induces CD4+ T helper cells or CD8+ T helper cells.
7. The composition of claim 4 , wherein the cytokine is a chicken cytokine.
8. The composition of claim 7 , wherein the chicken cytokine is chicken IFN-γ or chicken IL-12.
9. The composition of claim 1 , wherein said composition comprises a plurality of promiscuous T-cell epitopes.
10. The composition of claim 1 , wherein said antigen comprises SEQ ID NO: 148.
11. The composition of claim 1 , wherein said antigen comprises SEQ ID NO: 146.
12. The composition of claim 1 , further comprising a membrane protein OmpA1 from Aeromonas salmonicida , or a fragment thereof.
13. The composition of claim 12 , wherein the membrane protein OmpA1 comprises or consists of a 155 amino acid fragment of a membrane protein OmpA1.
14. The composition of claim 1 , wherein the promiscuous epitope is connected to the antigen by a spacer.
15. The composition of claim 1 , wherein at least one T-cell epitope is linked to another T-cell epitope by a linker comprising at least one glycine.
16. The composition of claim 1 , wherein the composition comprises the promiscuous T-cell, epitope.
17. A method for inducing an immune response in a host, comprising administering the composition of claim 1 to a fish.
18. A composition comprising a DNA molecule encoding a polypeptide, wherein the polypeptide comprises: (i) a truncated VP2 antigen of infectious pancreatic necrosis virus (IPNV) and (ii) optionally encoding a promiscuous T-cell epitope having the amino acid sequence selected from group consisting of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7 or a combination thereof, wherein said DNA molecule encoding the truncated VP2 antigen of IPNV comprises the nucleotide sequence of SEQ ID NO: 145 or SEQ ID NOI: 147.
19. The composition of claim 18 , wherein said antigen comprises SEQ ID NO: 148.
20. The composition of claim 18 , wherein the DNA molecule further comprises a nucleic acid sequence encoding a linker comprising at least one glycine, wherein the linker joins at least one T-cell epitope to another T-cell epitope.
21. The DNA molecule of claim 18 , further encoding a membrane protein OmpA1 from Aeromonas salmonicida , or a fragment thereof.
22. The DNA molecule of claim 21 , wherein the membrane protein OmpA1 comprises or consists of a 155 amino acid fragment of a membrane protein OmpA1.
23. A method for inducing an immune response comprises administering a composition of claim 18 into a host, wherein said composition comprises the DNA molecule encoding the truncated VP2 antigen of IPNV and the promiscuous T-cell epitope.
24. A method for inducing an immune response comprises administering a composition of claim 18 into a host, wherein said composition comprises the DNA molecule encoding the truncated VP2 antigen of IPNV.