Avians containing a lysozyme promoter transgene
View Patent ↗The invention provides for transgenic avians containing nucleic acids which include an exogenous lysozyme gene expression controlling nucleotide sequence which typically is linked to a polynucleotide encoding a heterologous polypeptide.
1. A transgenic chicken whose genome comprises an exogenous nucleic acid sequence encoding a protein operably linked to a functional fragment of the lysozyme promoter of SEQ ID NO: 67, wherein the chicken is capable of depositing the protein in egg white.
2. The transgenic chicken of claim 1 , wherein the protein is therapeutic.
3. The transgenic chicken of claim 1 , wherein the nucleic acid sequence comprises a vector.
4. The transgenic avian of claim 3 wherein the vector is selected from the group consisting of a plasmid, a viral vector and an artificial chromosome.
5. The transgenic chicken of claim 1 , wherein the functional fragment of the lysozyme promoter of SEQ ID NO: 67 is at least 95% identical to nucleotides 7665 to 11863 of SEQ ID NO: 67.
6. The transgenic chicken of claim 1 , wherein the functional fragment of the lysozyme promoter of SEQ ID NO: 67 is identical to nucleotides 7665 to 11863 of SEQ ID NO: 67.
7. The transgenic chicken of claim 1 , wherein the protein is human.
8. A method of producing protein in egg white of a transgenic chicken comprising obtaining a transgenic chicken whose genome comprises an exogenous nucleic acid sequence encoding a protein operably linked to a functional fragment of the lysozyme promoter of SEQ ID NO: 67, wherein the chicken is capable of depositing the protein in egg white; and
producing the protein in egg white of the transgenic chicken.
9. The method of claim 8 , wherein the functional fragment of the lysozyme promoter of SEQ ID NO: 67 is at least 95% identical to nucleotides 7665 to 11863 of SEQ ID NO: 67.
10. The method of claim 8 , wherein the functional fragment of the lysozyme promoter of SEQ ID NO: 67 is identical to nucleotides 7665 to 11863 of SEQ ID NO: 67.
11. The method of claim 8 , wherein the protein is human.