Glycosylated G-CSF obtained from a transgenic chicken
View Patent ↗Granulocyte colony stimulating factor obtained from eggs laid by transgenic avians having newly described G-CSF glycosylation patterns.
1. A composition comprising an isolated human G-CSF having a chicken-derived glycosylation pattern wherein the glycosylation pattern is other than that of G-CSF produced in a CHO cell.
2. An isolated human G-CSF having a chicken-derived glycosylation pattern wherein the glycosylation pattern is other than that of G-CSF produced in a CHO cell.
3. The composition of claim 1 wherein the G-CSF is present in a hard shell egg.
4. The composition of claim 1 wherein an G-CSF is glycosylated in an oviduct cell of an avian.
5. The composition of claim 4 wherein the oviduct cell is a tubular gland cell.
6. The composition of claim 1 wherein an G-CSF is glycosylated in an oviduct cell of a chicken.
7. The composition of claim 1 wherein the G-CSF is glycosylated at threonine 133.
8. The composition of claim 1 wherein the composition is a pharmaceutical composition.
9. The composition of claim 1 wherein the G-CSF has the amino acid sequence of SEQ ID NO: 41.
10. The composition of claim 1 wherein the glycosylation pattern is other than that of G-CSF produced in a human cell.