IP Library › Granted Patent US 7,807,365
Granted Patent B2
US 7,807,365 · App. 11/708,623 · Granted Oct 5, 2010

Cleavage of nucleic acids

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Quick Facts
Patent No.
US 7,807,365
App. No.
11/708,623
Granted
Oct 5, 2010
Kind
B2
Abstract

The invention relates to compositions and methods of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample.

Claims (24)

1. A method for characterizing a target nucleic acid molecule comprising :

a) exposing a sample comprising said target nucleic acid molecule to a composition comprising an isolated FEN-1 endonuclease, a probe nucleic acid and an isolated polymerase substantially lacking 5′ nuclease activity;

b) forming a cleavage structure under conditions such that said probe nucleic acid molecule hybridizes to said target nucleic acid;

c) altering said probe nucleic acid molecule with said isolated FEN-1 endonuclease; and

d) detecting the alteration of said probe nucleic acid molecule, thereby characterizing said target nucleic acid molecule.

2. The method of claim 1 , wherein said alteration comprises cleavage of said probe nucleic acid molecule.

3. The method of claim 2 , wherein said cleavage occurs by said FEN-1 endonuclease after said target nucleic acid molecule is reacted with said polymerase.

4. The method of claim 1 , wherein said exposing occurs under conditions permissive for primer extension by a polymerase.

5. The method of claim 4 , wherein said conditions permit exponential amplification of target nucleic acid molecule.

6. The method of claim 1 , wherein said target nucleic acid molecule is DNA.

7. The method of claim 1 , wherein said target nucleic acid molecule is RNA.

8. The method of claim 1 , wherein said target nucleic acid is cDNA.

9. The method of claim 1 , wherein said target nucleic acid molecule contains a nucleotide analogue.

10. The method of claim 1 , wherein said target nucleic acid molecule is from a pathogen.

11. The method of claim 1 , wherein said target nucleic acid is synthetic.

12. The method of claim 11 , wherein said target nucleic acid is produced by amplification.

13. The method of claim 12 , wherein said amplification comprises a polymerase chain reaction.

14. The method of claim 10 , wherein said pathogen is a virus.

15. The method of claim 10 , wherein said pathogen is a bacterium.

16. The method of claim 1 , wherein said sample is serum.

17. The method of claim 1 , wherein said sample is tissue.

18. The method of claim 1 , wherein said target nucleic acid molecule is attached to a solid support.

19. The method of claim 1 , wherein said probe nucleic acid molecule comprises a label.

20. The method of claim 19 , wherein said label comprises a fluorescent label.

Assignments (10)
RELEASE OF SECURITY INTEREST Recorded Apr 28, 2026
From: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
To: HOLOGIC, INC., ON ITS OWN BEHALF AND AS SUCCESSOR-BY-MERGER TO DIRECT RADIOGRAPHY CORP.; CYTYC CORPORATION, ON ITS OWN BEHALF AND AS SUCCESSOR-BY-MERGER TO BIOLUCENT, LLC; CYTYC SURGICAL PRODUCTS, LLC, AS SUCCESSOR-BY-CONVERSION TO CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; GEN-PROBE INCORPORATED, ON ITS OWN BEHALF AND AS SUCCESSOR-BY-MERGER TO THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE PRODESSE, INC.; SUROS SURGICAL SYSTEMS, INC.
Reel/Frame 075566/0039 →
CORRECTIVE ASSIGNMENT TO CORRECT THE INCORRECT PATENT NO. 8081301 PREVIOUSLY RECORDED AT REEL: 035820 FRAME: 0239. ASSIGNOR(S) HEREBY CONFIRMS THE SECURITY INTEREST RELEASE. Recorded Nov 9, 2017
From: GOLDMAN SACHS BANK USA, AS COLLATERAL AGENT
To: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
Reel/Frame 044727/0529 →
CORRECTIVE ASSIGNMENT TO CORRECT THE INCORRECT PATENT NO. 8081301 PREVIOUSLY RECORDED AT REEL: 028810 FRAME: 0745. ASSIGNOR(S) HEREBY CONFIRMS THE SECURITY AGREEMENT. Recorded Nov 9, 2017
From: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
To: GOLDMAN SACHS BANK USA
Reel/Frame 044432/0565 →
SECURITY AGREEMENT Recorded Aug 7, 2015
From: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; DIRECT RADIOGRAPHY CORP.; GEN-PROBE INCORPORATED; GEN-PROBE PRODESSE, INC.; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.
To: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
Reel/Frame 036307/0199 →
SECURITY INTEREST RELEASE REEL/FRAME 028810/0745 Recorded Jun 4, 2015
From: GOLDMAN SACHS BANK USA, AS COLLATERAL AGENT
To: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
Reel/Frame 035820/0239 →
SECURITY AGREEMENT Recorded Aug 1, 2012
From: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
To: GOLDMAN SACHS BANK USA
Reel/Frame 028810/0745 →
TERMINATION OF PATENT SECURITY AGREEMENTS AND RELEASE OF SECURITY INTERESTS Recorded Aug 26, 2010
From: GOLDMAN SACHS CREDIT PARTNERS, L.P., AS COLLATERAL AGENT
To: HOLOGIC, INC.; R2 TECHNOLOGY, INC.; SUROS SURGICAL SYSTEMS, INC.; BIOLUCENT, LLC; DIRECT RADIOGRAPHY CORP.; CYTYC SURGICAL PRODUCTS II LIMITED PARTNERSHIP; CYTYC SURGICAL PRODUCTS LIMITED PARTNERSHIP; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS III, INC.; CYTYC PRENATAL PRODUCTS CORP.; THIRD WAVE TECHNOLOGIES, INC.
Reel/Frame 024892/0001 →
EIGHTH SUPPLEMENT TO PATENT SECURITY AGREEMENT Recorded Mar 26, 2009
From: CYTYC CORPORATION; HOLOGIC, INC.
To: GOLDMAN SACHS CREDIT PARTNERSL.P.
Reel/Frame 022456/0947 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 15, 2008
From: STRATAGENE CALIFORNIA
To: HOLOGIC INC.
Reel/Frame 021976/0268 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 4, 2007
From: SORGE, JOSEPH A.
To: STRATAGENE CALIFORNIA
Reel/Frame 019409/0598 →