5-fluoro-uracil immunoassay
View Patent ↗Novel conjugates of 5-fluoro-uracil and novel 5-fluoro-uracil immunogens and monoclonal antibodies generated by these immunogens which are useful in immunoassays for the quantification and monitoring of 5-fluoro-uracil in biological fluids.
1. An antibody which binds selectively to 5-fluoro-uracil and has a cross-reactivity with each of uracil, cytosine and tegafur of not greater than 12%, said cross reactivity being relative to its binding to 5-fluoro-uracil.
2. The antibody of claim 1 , wherein said antibody is a monoclonal antibody.
3. The antibody of claim 2 , wherein said antibody is derived from mice, rabbits or rats.
4. The antibody of claim 1 , wherein said antibody is derived from an immunogenic carrier containing a polyamine polymer conjugated to a compound of the formula:
wherein Y is an organic spacing group;
X is a terminal functional group capable of binding to a polyamine polymer; and
p is an integer from 0 to 1.
5. The antibody of claim 4 , wherein said antibody is a monoclonal antibody.
6. The antibody of claim 5 , wherein said antibody is derived from mice, rabbits or rats.