Variant polynucleotide for increasing L-proline production
View Patent ↗The invention relates to mutated variants of the proB gene from coryneform bacteria, which encode γ-glutamyl kinase, and to processes for fermentative production of L-proline using bacteria which contain this mutation.
1. An isolated polynucleotide, which encodes a polypeptide comprising of SEQ ID NO: 4.
2. The isolated polynucleotide of claim 1 , which comprises SEQ ID NO:3.
3. A recombinant vector comprising the isolated polynucleotide of claim 1 .
4. A recombinant vector comprising the isolated polynucleotide of claim 2 .
5. A host cell comprising the isolated polynucleotide of claim 1 .
6. A host cell comprising the isolated polynucleotide of claim 2 .
7. The host cell of claim 5 , which is a Corynebacterium host cell.
8. The host cell of claim 6 , which is a Corynebacterium host cell.