Specimen container for the micro manipulation and biopsy in in-vitro fertilization
A container or dish is useful for the micro manipulation, micro injection, biopsy and fertilization in oocyte and embryo manipulation and culturing. The dish allows the user to more readily perform procedures used to fertilize oocytes by Intracytoplasmic sperm injection (ICSI) or other procedures, biopsy embryos and perform additional procedures for use in assisted reproductive techniques (ART), human reproduction and in-vitro fertilization (IVF). The invention allows ease in use, the reduction in the number of micro tools used in the procedure, as opposed to conventional dishes and procedures, and will allow the user to more readily locate the oocytes and embryos to be handled and worked on. The invention will add repetitiveness, consistency, improve efficacy and ease of procedure and may improve outcomes. The invention will also give the user a more ergonomically practical dish for these types of procedures and related protocols. Specimens other than oocytes and embryos can be treated in the container by means of micro manipulation.
1 . A mammalian cell specimen manipulation or biopsy apparatus comprising:
a) a container for holding the specimens, and for holding a culturing medium which sustains the specimens during the procedure, said container having a bottom wall; and
b) at least one specimen gathering structure in said container, said structure being positioned on said bottom wall of said container, and said structure being sized to capture single ones of the specimens.
2 . The apparatus of claim 1 wherein said structure is disposed between opposed downwardly and inwardly angled portions of said bottom wall, said portions being operative to direct specimens in said container toward said structure.
3 . The apparatus of claim 2 wherein said structure is a slot having a bottom surface and opposed side surfaces that intersect said portions of said container bottom wall.
4 . The apparatus of claim 3 wherein one of said opposed side surfaces is taller than the other.
5 . The apparatus of claim 3 further comprising a post structure on one of the angled portions of said bottom wall which post structure is operative to dislodge a specimen from a micro tool that is inserted into said container so that the dislodged specimen will settle into said specimen gathering structure in said container.
6 . The apparatus of claim 1 wherein said container is a dish having a side wall, a bottom wall and a top wall, and which includes a plurality of wells in said top wall, each of which wells containing a separate specimen gathering structure whereby multiple specimens can be segregated and treated in said container, one in each of said wells.