CYP1B1 nucleic acids and methods of use
The present invention provides nucleic acids containing transcriptional units that encode CYP1B1 polypeptides or portions thereof, wherein the transcriptional units lack sequences found in the untranslated region (UTR) of naturally occurring forms of the CYP1B1 transcript. The nucleic acids of the invention lack translational repressor elements and thus provide for a system of enhanced translation of the CYP1B1 polypeptide or portions thereof. Also disclosed are methods of administering nucleic acids to a mammal and use in the treatment of proliferative disorders or cancer.
1. A method of inducing an immune response in a human having a cancer, the method comprising administering to a human having a cancer an effective amount of a composition comprising an expression vector comprising a nucleic acid sequence encoding a polypeptide comprising a peptide sequence consisting of (i) an amino terminus methionine residue or targeting signal, and (ii) an amino acid sequence selected from the group consisting of SEQ ID NO:43, SEQ ID NO:47, and SEQ ID NO:50.
2. A method of inducing an immune response in a human having a cancer, the method comprising administering to a human having a cancer an effective amount of a composition comprising a microparticle comprising an expression vector comprising a nucleic acid sequence encoding a polypeptide comprising a peptide sequence consisting of (i) an amino terminus methionine residue or targeting signal, and (ii) an amino acid sequence selected from the group consisting of SEQ ID NO:43, SEQ ID NO:47, and SEQ ID NO:50.
3. The method of claim 1 , wherein the composition is administered intramuscularly.
4. The method of claim 1 , wherein the composition is administered subcutaneously.
5. The method of claim 1 , wherein the immune response is a T cell response.
6. The method of claim 1 , wherein the cancer is a cancer of the bladder, breast, colon, connective tissue, lung, esophagus, skin, lymph node, brain, ovary, stomach, uterus, testis, or prostate.
7. The method of claim 6 , wherein the cancer is a cancer of the colon.
8. The method of claim 6 , wherein the cancer is a cancer of the lymph node.
9. The method of claim 6 , wherein the cancer is a cancer of the ovary.
10. The method of claim 6 , wherein the cancer is a cancer of the prostate.
11. The method of claim 2 , wherein the composition is administered intramuscularly.
12. The method of claim 2 , wherein the composition is administered subcutaneously.
13. The method of claim 2 , wherein the immune response is a T cell response.
14. The method of claim 2 , wherein the cancer is a cancer of the bladder, breast, colon, connective tissue, lung, esophagus, skin, lymph node, brain, ovary, stomach, uterus, testis, or prostate.
15. The method of claim 14 , wherein the cancer is a cancer of the colon.
16. The method of claim 14 , wherein the cancer is a cancer of the lymph node.
17. The method of claim 14 , wherein the cancer is a cancer of the ovary.
18. The method of claim 14 , wherein the cancer is a cancer of the prostate.
19. The method of claim 2 , wherein the microparticle comprises a polymeric matrix comprising poly lactide-co-glycolide.
20. The method of claim 2 , wherein the microparticle comprises a lipid.
21. The method of claim 2 , wherein the polypeptide comprises SEQ ID NO: 43.
22. The method of claim 21 , wherein the composition is administered intramuscularly.
23. The method of claim 21 , wherein the composition is administered subcutaneously.
24. The method of claim 21 , wherein the cancer is a cancer of the bladder, breast, colon, connective tissue, lung, esophagus, skin, lymph node, brain, ovary, stomach, uterus, testis, or prostate.
25. The method of claim 24 , wherein the cancer is a cancer of the colon.
26. The method of claim 24 , wherein the cancer is a cancer of the lymph node.
27. The method of claim 24 , wherein the cancer is a cancer of the ovary.
28. The method of claim 24 , wherein the cancer is a cancer of the prostate.
29. The method of claim 2 , wherein the polypeptide comprises SEQ ID NO: 47.
30. The method of claim 29 , wherein the composition is administered intramuscularly.
31. The method of claim 29 , wherein the composition is administered subcutaneously.
32. The method of claim 21 , wherein the cancer is a cancer of the bladder, breast, colon, connective tissue, lung, esophagus, skin, lymph node, brain, ovary, stomach, uterus, testis, or prostate.
33. The method of claim 24 , wherein the cancer is a cancer of the colon.
34. The method of claim 24 , wherein the cancer is a cancer of the lymph node.
35. The method of claim 24 , wherein the cancer is a cancer of the ovary.
36. The method of claim 24 , wherein the cancer is a cancer of the prostate.