Method and apparatus for collecting biological materials

1. A system to separate a component from a selected material, comprising:

a separation container operable to contain the selected material having a top and a bottom;

a top wall at a proximal end of said separation container that closes the top of the separation container;

a piston positioned in said separation container between the top and the bottom of the separation container;

an injection port extending through the top wall;

a conduit positioned in said separation container operable to remove the selected material from a distal end near said bottom of said separation container between said piston and said bottom; and

a stop member at a fixed location within said separation container and operable to engage said piston at the fixed location within said separation container;

wherein said stop member is operable to resist a motion of said piston relative to said separation container.

2. The system of claim 1 , further comprising:

a centrifuge;

wherein said separation container is operable to be positioned in said centrifuge and said centrifuge is operable to spin said separation container;

wherein said piston is operable to move within said separation container between said top and said bottom due to a force on said piston during said spin in said centrifuge.

3. The system of claim 1 , wherein said conduit includes a tube extending between an extraction port defined in the top wall at the proximal end and said distal end of said separation container.

4. The system of claim 1 , wherein said piston includes a density of about 1.00 grams per milliliter to about 1.10 grams per milliliter.

5. The system of claim 1 , further comprising:

at least one of a collection system, a mixing system, an application system, a withdrawal system, or combinations thereof.

6. The system of claim 1 ,

wherein said stop member is connected to at least one of said conduit or said separation container and is operable to resist a motion of said piston relative to said separation container.

7. The system of claim 3 , wherein said piston is operable to move along said conduit; and

wherein said stop member defines a surface to engage said piston to stop a movement of said piston along said conduit.

8. The system of claim 1 , further comprising:

a port extending through said top wall;

wherein said conduit extends through said piston;

wherein said conduit and said port operably interconnect to allow withdrawal of the selected component from a position on a side of the piston opposite a side of the piston near of the top wall.

9. A system to separate a component from a selected material, comprising:

a container having a side wall, bottom wall, and a top wall and defining an interior volume;

an input port extending from the top wall and defining a first passage through said top wall to said interior volume;

an extraction port extending from the top wall;

a piston operable to move within said interior volume of said container due to a force exerted on said piston during the separation of the component from the selected material;

a conduit extending from said extraction port, including:

a tube extending from said top wall; and

a passage through said piston;

wherein the conduit allows extraction of the component from a position between said piston and said bottom wall; and

a stop member;

wherein the stop member is connected at a fixed location relative to at least one of said tube or said container;

wherein said stop member cooperates with said piston to limit a distance said piston is able to move within said interior volume of said container when said piston moves due to the force exerted on said piston during the separation of the component from the selected material.

10. The system of claim 9 , further comprising:

an input port extender;

wherein said input port extender defines a second passage operable with the first passage of the input port to allow access to said interior volume.

11. The system of claim 10 , further comprising:

an input syringe;

wherein said input syringe is operable to be interconnected with at least one of the input port or the input port extender;

wherein the syringe is operable to cooperate with at least one of the first passage, the second passage, or combinations thereof.

12. The system of claim 9 , wherein said input port and said first passage are operable to provide an access to said interior volume between said top wall and said piston.

13. The system of claim 9 , further comprising:

an extraction syringe, wherein the extraction syringe is operable to be interconnected with said extraction port.

14. The system of claim 13 , wherein said extraction syringe is operable with said conduit and said extraction port to extract the component from a position between said piston and said bottom wall after said piston has moved due to the force exerted on said piston during the separation of the component from the selected material.

15. A system to separate a component from a selected material, comprising:

a separation container operable to contain the selected material having a top and a bottom;

a top wall at the top of the separation container that closes the top of the separation container;

an injection portion extending through the top wall;

an extraction portion extending though the top wall;

a piston positioned in said separation container operable to move in an area between the top and the bottom of the separation container during a separation of the component from the selected material;

a conduit fixed in said separation container extending from the extraction portion at least towards the piston, wherein the conduit is operable to allow removal of the selected material from near the bottom of the separation container in an area between the piston and the bottom of the separation container; and

a stop member that is connected at a fixed location to at least one of the separation container or the conduit;

wherein said conduit includes a tube and a passage in said piston;

wherein said tube is operable to extend into said passage in said piston;

wherein said piston is operable to move along said tube;

wherein the stop member is operable to resist a motion of the piston relative to the separation container when the piston moves along said tube.

16. The system of claim 15 , further comprising:

an injection passage through the top wall whereby the injection portion is operable to allow access to an interior of the separation container; and

an extraction passage through the top wall whereby the extraction portion is operable to allow extraction from near the bottom of the separation container in an area between the piston and the bottom of the separation container.

17. The system of claim 15 , further comprising:

a centrifuge;

wherein the selected material is operable to be placed in the separation container between the piston and the top of the separation container;

wherein the piston is operable to move towards the top from near the bottom during centrifugation in the centrifuge;

wherein the component is operable to be withdrawn sequentially through the passage in the piston, the conduit, and the extraction portion.