Mixing bag with integral sparger and sensor receiver
A mixing bag for use in bioprocessing in which a fluid is received and agitated using an internal fluid-agitating element driven by an external motive device is disclosed. The bag may include an integral sparger and sensor receiver. Related methods are also disclosed.
1. A disposable bioprocessing apparatus intended for receiving a fluid in need of agitation and sparging using a gas, comprising:
a bag having a flexible sidewall forming an interior compartment capable of receiving and holding the fluid;
an integral sparger secured to the flexible sidewall of the bag for forming bubbles from the gas supplied to the fluid when present in the interior compartment; and
a fluid-agitating element for agitating the fluid and assisting in distributing the bubbles throughout the fluid when present in the interior compartment,
whereby the fluid-agitating element may be used to agitate the fluid while sparging is provided to the fluid by delivering the gas to the integral sparger to form bubbles distributed throughout the fluid by the fluid-agitating element in the course of bioprocessing involving the fluid when present in the bag.
2. The apparatus according to claim 1 , wherein the sparger includes a rigid base connected to a permeable or perforated film facing the interior compartment of the bag.
3. The apparatus according to claim 2 , wherein the sparger includes a conduit for coupling with a tube external to the interior compartment of the bag for supplying the gas to the fluid when present.
4. The apparatus according to claim 2 , wherein the sparger includes a dome-shaped portion.
5. The apparatus according to claim 2 , wherein the sparger includes a post extending inwardly into the interior compartment of the bag.
6. The apparatus according to claim 2 , further including a motive device exterior to the interior compartment of the bag for moving the fluid-agitating element within the bag.
7. The apparatus according to claim 2 , wherein the fluid-agitating element is rotatable about a central axis.
8. The apparatus according to claim 7 , wherein the sparger is annular and positioned in alignment with the central axis and adjacent to the fluid-agitating element to enhance the distribution of bubbles throughout the fluid.
9. A bioprocessing apparatus intended for receiving a fluid in need of agitation and sparging using a gas, comprising:
a bag having a flexible sidewall creating an interior compartment capable of receiving and holding the fluid;
an integral sparger connected to the bag and including a permeable material for forming bubbles from the gas supplied to the fluid when present in the bag; and
a fluid-agitating element for agitating the fluid and assisting in distributing the bubbles throughout the fluid when present in the interior compartment,
whereby the fluid-agitating element may be used to agitate the fluid while sparging is provided to the fluid by delivering the gas to the integral sparger to form bubbles distributed throughout the fluid by the fluid-agitating element in the course ofbioprocessing involving the fluid when present in the bag.
10. The apparatus according to claim 9 , wherein the sparger is welded to the bag to form a fluid-impervious seal.
11. The apparatus according to claim 9 , wherein the permeable material comprises a gas permeable film, whereby the gas passing through the film forms the bubbles distributed through the fluid in the interior compartment by the fluid-agitating element.
12. The apparatus according to claim 9 , wherein the sparger comprises a rigid base having a peripheral flange for connecting to the flexible sidewall of the bag.
13. The apparatus according to claim 9 , wherein the sparger includes a conduit projecting external to the interior compartment for supplying the gas to the fluid when present in the interior compartment.
14. The apparatus according to claim 9 , wherein the fluid-agitating element comprises an annular body having an opening associated with a post connected to the bag.
15. A disposable hioprocessing apparatus intended for receiving a fluid in need of agitation and sparging using a gas, comprising:
a flexible bag having a sidewall forming an interior compartment capable of receiving and holding the fluid;
an integral sparger comprising a rigid post projecting inwardly into the interior compartment of the bag, said post having a passage for receiving the gas that forms bubbles in the fluid when present in the interior compartment; and
a fluid-agitating element associated with the post for agitating the fluid and assisting in distributing the bubbles throughout the fluid when present in the interior compartment,
whereby the fluid-agitating element may be used to agitate the fluid while sparging is provided to the fluid by delivering the gas to the integral sparger to form bubbles distributed throughout the fluid by the fluid-agitating element in the course of bioprocessmg involving the fluid when present in the bag.
16. The apparatus of claim 15 , wherein the fluid-agitating element comprises a magnet and at least one blade for assisting in agitating the fluid.
17. The apparatus of claim 15 , wherein the sparger comprises a perforated material for forming bubbles from the gas.
18. The apparatus of claim i 5 , further including a peripheral flange connected to the post of the sparger.
19. The apparatus of claim 15 , wherein the sparger and the fluid-agitating element are generally concentric.
20. The apparatus of claim 15 , wherein the fluid-agitating element is rotatably positioned in the interior compartment of the bag.
21. The apparatus according to claim 20 , further including a motive device exterior to the interior compartment of the bag for rotating the fluid-agitating element.