Intracellular kinase inhibitors
View Patent ↗Intracellular kinase inhibitors and their therapeutic uses for patients with T cell malignancies, B cell malignancies, autoimmune disorders, and transplanted organs.
1. A protein kinase inhibitor selected from the group consisting of:
(a) compounds of structural formula II
wherein:
R 3 , R 4 , R 5 , and R 6 are independently hydrogen or optionally substituted C 1 -C 6 alkyl;
R 9 is selected from,
R 10 is hydrogen, —OH, —COOH, —CONH 2 , or —NCO;
(b) compounds of structural formula III:
wherein:
R 3 , R 4 , R 5 , and R 6 are independently hydrogen or optionally substituted C 1 -C 6 alkyl;
R 11 and R 12 are independently selected from hydrogen, —OCH 3 , halogen, —NO 2 , —CN, —CF 3 , —NCOR′ (wherein R′ is hydrogen or C 1 -C 4 alkyl), phenyloxy, —OCF 3 , —NR′R″ (wherein R′ and R″ are independently hydrogen or C 1 -C 4 alkyl), C 1 -C 4 alkyl, C 1 -C 4 alkoxy, and —SO 2 R′ (wherein R′ is hydrogen or C 1 -C 4 alkyl); and
R 13 is hydrogen, C 1 -C 4 alkyl,
(c) compounds of structural formula IV:
wherein R 1 and R 2 (a) are independently hydrogen, optionally substituted C 1 -C 6 alkyl, piperidine, or furanyl; or (b) are taken together with the nitrogen atom to which they are attached to form (i) a 5- to 7-membered optionally substituted aryl, (ii) a 5- to 7-membered optionally substituted heteroaryl, or (iii) a 5- to 7-membered optionally substituted heterocycle which may be unfused or fused to an optionally substituted aryl;
(d) compounds of structural formula VI:
wherein:
R 3 , R 4 , R 5 , and R 6 are independently hydrogen or optionally substituted C 1 -C 6 alkyl; and
R 10 is hydrogen, —OH, —COOH, —CONH 2 , or —NCO;
(e) compounds of structural formula VI:
wherein:
R 1 , R 2 , R 3 , R 4 , R 5 , and R 6 are as defined above;
R 14 is hydrogen or ═O; and
D is CH or NH;
(f) compounds of structural formula VII:
wherein:
R 3 , R 4 , R 5 , and R 6 are independently hydrogen or optionally substituted C 1 -C 6 alkyl; and
R 1 and R 2 are independently hydrogen, C 1 -C 4 alkyl,
(wherein R 15 is halogen or C 1 -C 4 alkyl and R 16 is C 1 -C 4 alkyl), or R 1 and R 2 together with the nitrogen to which they are attached form an aryl group selected from
(wherein R 17 and R 18 are independently hydrogen or —OCH 3 ,)
(wherein R 1 and R 2 are independently hydrogen or C 1 -C 4 alkyl)
(wherein n=1-4), and phenyl-C 1 -C 4 alkyl, optionally substituted with halogen;
(g) compounds of structural formula VIII:
or a pharmaceutically acceptable salt thereof, wherein R 3 , R 4 , R 5 , and R 6 are independently hydrogen or optionally substituted C 1 -C 6 alkyl;
(h) compounds of structural formula IX:
wherein:
R 3 , R 4 , R 5 , and R 6 are independently hydrogen or optionally substituted C 1 -C 6 alkyl; and either
R 1 is hydrogen and R 2 is
(wherein R 19 is selected from hydrogen and
R 1 and R 2 together with the nitrogen to which they are attached are
A is N or O;
R 20 is phenyl-C 1 -C 4 alkyl optionally substituted with one or more halogens, hydrogen, C 1 -C 4 alkyl, amino-C 1 -C 4 alkyl,
R 17 and R 18 are independently hydrogen or —OCH 3 ;
R 21 is —CONR′R″, —COR′,
R′ and R″ are independently selected from hydrogen and C 1 -C 4 alkyl; and
(i) compounds of structural formula XI:
wherein:
R 3 , R 4 , R 5 , and R 6 are independently hydrogen or optionally substituted C 1 -C 6 alkyl;
G is N, CH, or S;
G′ is NH, CH, or S;
R 23 is hydrogen, —NR′R″ C 1 -C 4 linear alkyl, C 1 -C 4 alkyl, phenyl-C 1 -C 4 alkyl, —CONH 2 , or —CO R′R″; and
R′ and R″ are independently selected from hydrogen and C 1 -C 4 alkyl, wherein the protein kinase inhibitor (1) inhibits interleukin-2 inducible tyrosine kinase (ITK) with an IC 50 of 0.00085 μM-1 μM in an in vitro kinase assay; (2) inhibits Bruton's tyrosine kinase (BTK) with an IC 50 of 0.00072 μM-1 μM in an in vitro kinase assay; or (3) inhibits interleukin-2 inducible tyrosine kinase (ITK) with an IC 50 of 0.00085 μM-1 μM and inhibits Bruton's tyrosine kinase (BTK) with an IC 50 of 0.00072 μM-1 μM in an in vitro kinase assay, with the proviso the protein kinase inhibitor is not
2. The protein kinase inhibitor of claim 1 which is a compound of structural formula II.
3. The protein kinase inhibitor of claim 1 which is a compound of structural formula III.
4. The protein kinase inhibitor of claim 1 which is a compound of structural formula IV.
5. The protein kinase inhibitor of claim 1 which is a compound of structural formula V.
6. The protein kinase inhibitor of claim 1 which is a compound of structural formula VI.
7. The protein kinase inhibitor of claim 1 which is a compound of structural formula VII.
8. The protein kinase inhibitor of claim 1 which is a compound of structural formula VIII.
9. The protein kinase inhibitor of claim 1 which is a compound of structural formula IX.
10. The protein kinase inhibitor of claim 1 which is a compound of structural formula XI.
11. A composition comprising:
(a) a pharmaceutically acceptable vehicle; and
(b) a protein kinase inhibitor of claim 1 .
12. The composition of claim 11 , wherein the protein kinase inhibitor is a compound of structural formula II.
13. The composition of claim 11 , wherein the protein kinase inhibitor is a compound of structural formula III.
14. The composition of claim 11 , wherein the protein kinase inhibitor is a compound of structural formula IV.
15. The composition of claim 11 , wherein the protein kinase inhibitor is a compound of structural formula V.
16. The composition of claim 11 , wherein the protein kinase inhibitor is a compound of structural formula VI.
17. The composition of claim 11 , wherein the protein kinase inhibitor is a compound of structural formula VII.
18. The composition of claim 11 , wherein the protein kinase inhibitor is a compound of structural formula VIII.
19. The composition of claim 11 , wherein the protein kinase inhibitor is a compound of structural formula IX.
20. The composition of claim 11 , wherein the protein kinase inhibitor is a compound of structural formula XI.
21. An adduct comprising:
(a) a protein kinase inhibitor of claim 1 ; and
(b) an aspartate-lysine-cysteine (DKC) triad kinase domain.
22. The adduct of claim 21 wherein the DKC triad kinase domain is an ITK kinase domain.
23. The adduct of claim 21 wherein the DKC triad kinase domain is a BTK kinase domain.
24. The adduct of claim 21 , wherein the protein kinase inhibitor is a compound of structural formula II.
25. The adduct of claim 21 , wherein the protein kinase inhibitor is a compound of structural formula III.
26. The adduct of claim 21 , wherein the protein kinase inhibitor is a compound of structural formula IV.
27. The adduct of claim 21 , wherein the protein kinase inhibitor is a compound of structural formula V.
28. The adduct of claim 21 , wherein the protein kinase inhibitor is a compound of structural formula VI.
29. The adduct of claim 21 , wherein the protein kinase inhibitor is a compound of structural formula VII.
30. The adduct of claim 21 , wherein the protein kinase inhibitor is a compound of structural formula VIII.
31. The adduct of claim 21 , wherein the protein kinase inhibitor is a compound of structural formula IX.
32. The adduct of claim 21 , wherein the protein kinase inhibitor is a compound of structural formula XI.
33. A complex comprising a protein kinase inhibitor of claim 1 which is bound to an aspartate-lysine-cysteine (DKC) triad kinase.
34. The complex of claim 33 wherein the DKC triad kinase is ITK.
35. The complex of claim 33 wherein the DKC triad kinase is BTK.
36. The complex of claim 33 which consists of the protein kinase inhibitor bound to the DKC triad kinase.
37. The complex of claim 33 , wherein the protein kinase inhibitor is a compound of structural formula II.
38. The complex of claim 33 , wherein the protein kinase inhibitor is a compound of structural formula III.
39. The complex of claim 33 , wherein the protein kinase inhibitor is a compound of structural formula IV.
40. The complex of claim 33 , wherein the protein kinase inhibitor is a compound of structural formula V.
41. The complex of claim 33 , wherein the protein kinase inhibitor is a compound of structural formula VI.
42. The complex of claim 33 , wherein the protein kinase inhibitor is a compound of structural formula VII.
43. The complex of claim 33 , wherein the protein kinase inhibitor is a compound of structural formula VIII.
44. The complex of claim 33 , wherein the protein kinase inhibitor is a compound of structural formula IX.
45. The complex of claim 33 , wherein the protein kinase inhibitor is a compound of structural formula XI.
46. A method of inhibiting kinase activity, comprising contacting an aspartate-lysine-cysteine (DKC) triad kinase with a protein kinase inhibitor of claim 1 or a pharmaceutically acceptable salt thereof, whereby kinase activity of the DKC triad kinase is inhibited.
47. The method of claim 46 wherein the contacting occurs in a cell-free system.
48. The method of claim 46 wherein the contacting occurs in a cell.
49. The method of claim 48 wherein the cell is in vitro.
50. The method of claim 48 wherein the cell is in a patient.
51. The method of claim 50 wherein patient has an organ transplant, an autoimmune disease, or a blood cell malignancy.
52. The method of claim 46 wherein the DKC triad kinase is ITK.
53. The method of claim 46 wherein the DKC triad kinase is BTK.
54. The method of claim 46 wherein the protein kinase inhibitor has structural formula II.
55. The method of claim 46 wherein the protein kinase inhibitor has structural formula III.
56. The method of claim 46 wherein the protein kinase inhibitor has structural formula IV.
57. The method of claim 46 wherein the protein kinase inhibitor has structural formula V.
58. The method of claim 46 wherein the protein kinase inhibitor has structural formula VI.
59. The method of claim 46 wherein the protein kinase inhibitor has structural formula VII.
60. The method of claim 46 wherein the protein kinase inhibitor has structural formula VIII.
61. The method of claim 46 wherein the protein kinase inhibitor has structural formula IX.
62. The method of claim 46 wherein the protein kinase inhibitor has structural formula XI.