CPC214 gene promoter sequences and methods of use
View Patent ↗The present invention relates to polynucleotide molecules for regulating gene expression in plants. In particular, the invention relates to CPC214 promoters isolated from Zea mays that are useful for regulating gene expression of heterologous polynucleotide molecules in plants. The invention also relates to expression constructs and transgenic plants containing the heterologous polynucleotide molecules.
1. A polynucleotide molecule comprising a sequence selected from the group consisting of SEQ ID NOs: 18, 19, 20, or a fragment of at least 50 contiguous nucleotides thereof having promoter activity, wherein said polynucleotide molecule is operably linked to a heterologous transcribable nucleic acid.
2. The polynucleotide molecule of claim 1 , wherein said polynucleotide molecule comprises the polynucleotide sequence of SEQ ID NO: 18.
3. The polynucleotide molecule of claim 1 , wherein said polynucleotide molecule comprises the polynucleotide sequence of SEQ ID NO: 19.
4. The polynucleotide molecule of claim 1 , wherein said polynucleotide molecule comprises the polynucleotide sequence of SEQ ID NO: 20.
5. An expression vector comprising the polynucleotide molecule of claim 1 .
6. The polynucleotide molecule of claim 1 , wherein said heterologous transcribable nucleic acid is a nucleic acid of agronomic interest.
7. The polynucleotide molecule of claim 1 , wherein said heterologous transcribable nucleic acid is a marker gene.
8. A transgenic plant stably transformed with the polynucleotide molecule of claim 1 .
9. The transgenic plant of claim 8 , wherein said plant is a monocotyledon selected from the group consisting of wheat, maize, rye, rice, oat, barley, turfgrass, sorghum, millet and sugarcane.
10. The transgenic plant of claim 8 , wherein said transcribable nucleic acid confers altered cell proliferation in the embryo, aleurone, or both to said transgenic plant.
11. The transgenic plant of claim 8 , wherein said transcribable nucleic acid confers altered oil content in the embryo, aleurone, or both to said transgenic plant.
12. The transgenic plant of claim 8 , wherein said transcribable nucleic acid confers altered protein quality in the embryo, aleurone, or both to said transgenic plant.
13. The transgenic plant of claim 8 , wherein said transcribable nucleic acid confers altered micronutrient content to said transgenic plant.
14. A seed of the transgenic plant of claim 8 , wherein said seed comprises said polynucleotide molecule.
15. A part of the transgenic plant of claim 8 , wherein said part comprises said polynucleotide molecule.
16. The part of claim 15 , further defined as a cell of the transgenic plant of claim 8 , wherein said cell comprises said polynucleotide molecule.
17. A method of producing a vegetable oil, comprising the steps of:
a) incorporating in the genome of an oilseed producing plant the polynucleotide molecule of claim 1 ; wherein said transcribable nucleic acid confers altered oil content to said oilseed;
b) growing the plant to produce oil; and
c) extracting the oil from seeds of the plant.
18. A method of making protein, comprising the steps of:
a) obtaining the transgenic plant of claim 8 ;
b) growing the plant to produce protein; and
c) extracting protein from the plant.
19. The polynucleotide molecule of claim 1 , wherein said polynucleotide molecule comprises a fragment of at least 90 contiguous nucleotides of SEQ ID NO:18 having promoter activity.
20. The polynucleotide molecule of claim 1 , wherein said polynucleotide molecule comprises a fragment of at least 200 contiguous nucleotides of SEQ ID NO:18 having promoter activity.
21. The transgenic plant of claim 8 , wherein said polynucleotide molecule comprises SEQ ID NO:18.
22. The transgenic plant of claim 8 , wherein said polynucleotide molecule comprises SEQ ID NO:19.
23. The transgenic plant of claim 8 , wherein said polynucleotide molecule comprises SEQ ID NO:20.
24. The transgenic plant of claim 8 , wherein said polynucleotide molecule comprises a fragment of at least 90 contiguous nucleotides of SEQ ID NO:18 having promoter activity.
25. The transgenic plant of claim 8 , wherein said polynucleotide molecule comprises a fragment of at least 200 contiguous nucleotides of SEQ ID NO:18 having promoter activity.
26. The seed of claim 14 , wherein said polynucleotide molecule comprises SEQ ID NO:18.
27. The seed of claim 14 , wherein said polynucleotide molecule comprises SEQ ID NO:19.
28. The seed of claim 14 , wherein said polynucleotide molecule comprises SEQ ID NO:20.
29. The seed of claim 14 , wherein said polynucleotide molecule comprises a fragment of at least 90 contiguous nucleotides of SEQ ID NO:18 having promoter activity.
30. The seed of claim 14 , wherein said polynucleotide molecule comprises a fragment of at least 200 contiguous nucleotides of SEQ ID NO:18 having promoter activity.
31. The part of claim 15 , wherein said polynucleotide molecule comprises SEQ ID NO:18.
32. The part of claim 15 , wherein said polynucleotide molecule comprises SEQ ID NO:19.
33. The part of claim 15 , wherein said polynucleotide molecule comprises SEQ ID NO:20.
34. The part of claim 15 , wherein said polynucleotide molecule comprises a fragment of at least 90 contiguous nucleotides of SEQ ID NO:18 having promoter activity.
35. The part of claim 15 , wherein said polynucleotide molecule comprises a fragment of at least 200 contiguous nucleotides of SEQ ID NO:18 having promoter activity.