Labeled solid supports for organic synthesis
View Patent ↗The disclosed invention pertains to improved oligonucleotide manufacturing methods, including novel support compositions that are optionally labeled, their methods of preparation and use. The compositions and methods are particularly well suited for high throughput oligonucleotide manufacturing in that the automated support recognition facilitates loading of the wells with the proper supports. In addition, the labeled supports can be used to confirm that each well of a multi-well plate, such as a 96 or 384 well plate, was properly loaded.
1. A composition for performing oligonucleotide synthesis comprising a mixture of controlled pore glass and a thermoplastic polymeric material, said thermoplastic polymeric material comprising powder or beads or a mixture of powder and beads, wherein the mixture is heated to form a membrane.
2. The composition of claim 1 wherein the thermoplastic polymer is a polyene.
3. The composition of claim 2 wherein the polyene is a polyethylene or a polypropylene.
4. The composition of claim 1 further comprising a component that is capable of distinguishing said composition for performing oligonucleotide synthesis from a second composition for performing oligonucleotide synthesis, said second composition having an attached functional group that is different from a functional group attached to the first composition.
5. The composition of claim 4 wherein the component is a colored polymer.
6. The composition of claim 4 wherein the component is a dye.
7. The composition of claim 4 wherein the component is comprised of one or more magnetic beads.
8. The composition of claim 4 wherein the component is an inert filler.
9. A composition for oligonucleotide synthesis, comprising a support for oligonucleotide synthesis with zero, one or more functional groups attached to said support's surface, wherein the support is comprised of ultra high molecular weight polyethylene powder or beads and controlled pore glass.
10. The composition of claim 9 wherein the composition also comprises a tagging component to identify functional groups.
11. The composition of claim 10 wherein the tagging component is a colored polymer.
12. The compositions of claim 9 wherein the composition also comprises a tagging component to identify a scale of loading.
13. The compositions of claims 8 , 9 or 11 wherein the support also contains a linear low molecular weight polyethylene.
14. A composition for biopolymer synthesis comprising a synthesis support and a colored particle, said colored particle corresponding to a specific functional group derivatized to the support.
15. The composition of claim 9 wherein the composition also comprises a tagging component to differentiate between a set of supports with different universal linkers.
16. The composition of claim 1 wherein the thermoplastic polymeric material serves as a tagging component.
17. The composition of claim 5 wherein the colored polymer comprises less than 3% of the total composition.
18. The composition of claim 1 wherein the controlled pore glass and the thermoplastic polymeric material are in a biopolymer synthesis column.