Rebaudioside A composition and method for purifying rebaudioside A
View Patent ↗Exemplary embodiments of this invention encompass a method for purifying crude rebaudioside A. In particular, this invention relates to a method for purifying crude rebaudioside A compositions comprising purities from approximately 40% to approximately 95% rebaudioside A to obtain a substantially pure rebaudioside A product with a single crystallization step. Resulting polymorph and amorphous forms of rebaudioside A and methods for preparing polymorph and amorphous forms of rebaudioside A from crude rebaudioside A compositions and substantially pure rebaudioside A compositions also are disclosed.
1. A method for purifying polymorphic and/or amorphous forms of rebaudioside A comprising the steps of:
combining a crude rebaudioside A mixture containing an extract from Stevia plants with an aqueous organic solvent to form a rebaudioside A solution,
wherein the crude rebaudioside A mixture contains rebaudioside A in a purity from about 60% to about 85%, the aqueous organic solvent and the crude rebaudioside A mixture are present in the rebaudioside A solution in a weight ratio from about 4 to about 10 parts aqueous organic solvent to about 1 part crude rebaudioside A, and the aqueous organic solvent contains water in an amount from about 10% to about 25% by weight, and a mixture of ethanol and methanol;
heating the rebaudioside A solution at a temperature in a range from room temperature to about 40° C.;
crystallizing the rebaudioside A solution in a single crystallization step to provide a substantially pure rebaudioside A composition; and
separating and washing the substantially pure rebaudioside A composition to provide rebaudioside A in a purity greater than 95% by weight on a dry basis.
2. The method of claim 1 , wherein the crude rebaudioside A mixture contains rebaudioside A in a purity from about 70% to about 85% by weight.
3. The method of claim 1 , wherein the aqueous organic solvent and the crude rebaudioside A mixture are present in the rebaudioside A solution in a weight ratio from about 3 to about 5 parts aqueous organic solvent to about 1 part crude rebaudioside A.
4. The method of claim 1 , wherein the water content of the aqueous organic solvent ranges from about 15% to about 20% by weight.
5. The method of claim 1 , wherein the ethanol and methanol are present in the aqueous organic solution in a weight ratio from about 20 parts to about 1 part ethanol to about 1 part methanol.
6. The method of claim 1 , wherein the ethanol and methanol are present in the aqueous organic solution in a weight ratio from about 3 parts to about 1 part ethanol to about 1 part methanol.
7. The method of claim 1 , further comprising the step of cooling the rebaudioside A solution after the heating step.
8. The method of claim 7 , wherein the step of cooling the rebaudioside A solution comprises cooling the rebaudioside A solution for about 0.5 hours to about 24 hours.
9. The method of claim 1 , further comprising the step of drying the rebaudioside A having a purity greater than 95% by weight on a dry basis, wherein an anhydrous form of rebaudioside A results with drying.
10. The method of claim 1 , further comprising the steps of:
cooling the rebaudioside A solution after the heating step; and
drying the rebaudioside A having a purity greater than 95% by weight on a dry basis.
11. The method of claim 1 , wherein the rebaudioside A solution in the single crystallization step is stirred or unstirred.
12. The method of claim 1 , wherein the crude rebaudioside A contains substantially no rebaudioside D impurity, and the method further comprises slurrying the substantially pure rebaudioside A composition in an aqueous organic solvent or in an organic solvent.
13. The method of claim 1 , wherein the purity of rebaudioside A is greater than 97% by weight on a dry basis.
14. The method of claim 1 , wherein the purity of rebaudioside A is greater than 98% by weight on a dry basis.
15. The method of claim 1 , wherein the purity of rebaudioside A is greater than 99% by weight on a dry basis.