Tagging reagents and methods for hydroxylated compounds
View Patent ↗In various aspects, the present teachings provide labeling reagents and sets of labeling reagents for the relative quantitation, absolute quantitation, or both, of hydroxylated compounds including, but not limited to, hydroxylated ring containing compounds, steroids and sterols. In various aspects, the present teachings also provide methods for the analysis hydroxylated compounds including, but not limited to, hydroxylated ring containing compounds, steroids and sterols my MS/MS methods.
1. A set of isobaric labels comprising two or more compounds of the general formula (XIV):
or a salt or hydrate form thereof, wherein each of the isobaric labels of the set of isobaric labels contains one or more heavy atom isotopes.
2. The set of labels of claim 1 , wherein each label of the set of labels is isotopically enriched with two or more heavy atom isotopes.
3. The set of labels of claim 1 , wherein each label of the set of labels is isotopically enriched with three or more heavy atom isotopes.
4. The set of labels of claim 1 , wherein each label of the set of labels is isotopically enriched with four or more heavy atom isotopes.
5. The set of labels of claim 1 , wherein the heavy atom isotopes are each independently 13 C, 15 N, 18 O.
6. The set of labels of claim 1 , wherein the two or more compounds of the general formula (I) are in the form of a mono-TFA salt, a mono HCl salt, a bis-HCl salt, or a bis-TFA salt, or a hydrate thereof.
7. A set of isobaric labels comprising two or more N-substituted piperidine, piperazine or morpholine compounds of the general formula (XVII):
or a salt or hydrate form thereof, wherein each of the isobaric labels of the set of isobaric labels contains one or more heavy atom isotopes, and wherein,
R 10 represents C, N or O;
R 4 represents hydrogen, a straight chain or branched C 1 -C 6 alkyl group, or a straight chain or branched C 1 -C 6 alkoxy group; and
each R 5 represents independently hydrogen, deuterium, fluorine, chlorine, bromine, iodine, an amino acid side chain, a straight chain or branched C 1 -C 6 alkyl group that may optionally contain a substituted or unsubstituted aryl group wherein the carbon atoms of the alkyl and aryl groups each independently are bonded to one or more hydrogen, deuterium or fluorine atoms.
8. The set of labels of claim 7 , wherein each label of the set of labels is isotopically enriched with four or more heavy atom isotopes.
9. The set of labels of claim 7 , wherein the heavy atom isotopes are each independently 13 C, 15 N, 18 O.
10. The set of labels of claim 7 , wherein R 12 represents ═C═O.
11. The set of labels of claim 7 , wherein R 10 represents nitrogen.
12. The set of labels of claim 7 , wherein R 4 represents methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, or tert-butyl.
13. The set of labels of claim 7 , wherein each R 5 represents independently hydrogen, methyl or methoxy.
14. The set of labels of claim 7 , wherein the two or more N-substituted piperidine, piperazine or morpholine compounds are of the general formula (XVIII):
15. The set of labels of claim 14 , wherein R 4 is methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, or tert-butyl.
16. A set of labels comprising two or more compounds of the general formula (I):
Z—R 1 (I),
or a salt or hydrate form thereof, wherein one or more of the compounds in the set of labels contains one or more heavy atom isotopes and wherein,
Z represents a substituted or unsubstituted straight, branched or cyclic alkyl; a substituted or unsubstituted aryl; a substituted or unsubstituted hetero aryl; a substituted or unsubstituted amino; or a substituted thio;
wherein the two or more compounds are of the general formula (V):
or a salt or a hydrate form thereof, wherein:
R 1 represents
R 2 represents a substituted or unsubstituted alkyl; a substituted or unsubstituted halogenated alkyl; or a substituted or unsubstituted aryl,
R 3 represents C, N, O or S;
R 4 represents hydrogen, a straight chain or branched C 1 -C 6 alkyl group, or a straight chain or branched C 1 -C 6 alkoxy group; and
each R 5 represent independently hydrogen, deuterium, fluorine, chlorine, bromine, iodine, an amino acid side chain, a straight chain or branched C 1 -C 6 alkyl group that may optionally contain a substituted or unsubstituted aryl group wherein the carbon atoms of the alkyl and aryl groups each independently are bonded to one or more hydrogen, deuterium or fluorine atoms.
17. The set of labels of claim 16 , wherein the labels are isobaric in the unsalted or unhydrated form, and each of the isobaric labels contains one or more heavy atom isotopes.
18. The set of labels of claim 16 , wherein the masses of the labels differ by less than about 0.05 amu in the unsalted or unhydrated form.
19. A set of labels comprising two or more compounds of the general formula (I):
Z—R 1 (I),
or a salt or hydrate form thereof, wherein one or more of the compounds in the set of labels contains one or more heavy atom isotopes and wherein,
Z represents a substituted or unsubstituted straight, branched or cyclic alkyl; a substituted or unsubstituted aryl; a substituted or unsubstituted hetero aryl; a substituted or unsubstituted amino; or a substituted thio;
wherein the two or more compounds are of the general formula (IV)
or a salt or hydrate form thereof;
R 1 represents
and
R 2 represents a substituted or unsubstituted alkyl; a substituted or unsubstituted halogenated alkyl; or a substituted or unsubstituted aryl.
20. A set of labels comprising two or more compounds of the general formula (I):
Z—R 1 (I),
or a salt or hydrate form thereof, wherein one or more of the compounds in the set of labels contains one or more heavy atom isotopes and wherein,
Z represents a substituted or unsubstituted straight, branched or cyclic alkyl; a substituted or unsubstituted aryl; a substituted or unsubstituted hetero aryl; a substituted or unsubstituted amino; or a substituted thio;
wherein the two or more compounds are of the general formula (V):
or a salt or a hydrate form thereof, wherein:
R 1 represents
R 2 represents a substituted or unsubstituted alkyl; a substituted or unsubstituted halogenated alkyl; or a substituted or unsubstituted aryl;
R 3 represents C, N, O or S;
R 4 represents hydrogen, a straight chain or branched C 1 -C 6 alkyl group, or a straight chain or branched C 1 -C 6 alkoxy group; and
each R 5 represent independently hydrogen, deuterium, fluorine, chlorine, bromine, iodine, an amino acid side chain, a straight chain or branched C 1 -C 6 alkyl group that may optionally contain a substituted or unsubstituted aryl group wherein the carbon atoms of the alkyl and aryl groups each independently are bonded to one or more hydrogen, deuterium or fluorine atoms.
21. The set of labels of claim 20 , wherein each label in the set of labels being differentiated from the another label in the set by a mass difference greater than about 1 amu.
22. A set of labels comprising two or more N-substituted piperidine, piperazine or morpholine compounds of the general formula (XV):
or a salt or a hydrate form thereof, wherein:
R 9 represents —OC(═O)R 11 , where R 11 is a straight chain or branched alkyl group;
R 10 represents C, N or O;
R 4 represents hydrogen, a straight chain or branched C 1 -C 6 alkyl group, or a straight chain or branched C 1 -C 6 alkoxy group; and
each R 5 represents independently hydrogen, deuterium, fluorine, chlorine, bromine, iodine, an amino acid side chain, a straight chain or branched C 1 -C 6 alkyl group that may optionally contain a substituted or unsubstituted aryl group wherein the carbon atoms of the alkyl and aryl groups each independently are bonded to one or more hydrogen, deuterium or fluorine atoms;
wherein one or more of the compounds in the set of tags contains one or more heavy atom isotopes.