High Throughput Collagen Synthesis Assay
The present invention provides for a new plate-based high throughput screening of collagen synthesis wherein the collagen is left intact in the cells. The present invention also provides for collagen synthesis assay methods, methods of identifying candidate compounds which modulate collagen synthesis, and collagen synthesis assay compositions useful for modeling collagen depositions disorders, comprising idiopathic pulmonary fibrosis, liver fibrosis, renal fibrosis, heart fibrosis, rheumatoid arthritis, and atherosclerotic plaques.
1 . A collagen synthesis assay method, comprising:
intermixing collagen producing cells grown to confluency in the presence of a candidate compound, a fixing agent, a solvent and a collagen complexing dye;
determining whether said candidate compound modulates collagen synthesis; and
wherein said collagen is left intact in said cells.
2 . The method of claim 1 , wherein said cells are grown to post-confluency.
3 . The method of claim 1 , wherein said determining is done visually or colorimetrically using instruments selected from the group consisting of a spectrophotometer, colorimeter, spectroradiometer, densitometer, and microwell plate reader.
4 . The method of claim 1 , wherein said assay is used as a model for collagen deposition disorders selected from the group consisting of idiopathic pulmonary fibrosis, liver fibrosis, renal fibrosis, heart fibrosis, rheumatoid arthritis, and atherosclerotic plaques.
5 . The method of claim 1 , wherein said assay is used in a post-transplant patient.
6 . A method of identifying a candidate compound which modulates collagen synthesis, comprising:
growing collagen producing cells to confluency in the presence of said candidate compound;
assaying the mixture to determine whether said candidate compound modulates collagen synthesis; and
wherein said collagen is left intact in said cells.
7 . The method of claim 6 , wherein said cells are grown to post-confluency.
8 . The method of claim 6 , wherein said determining is done visually or colorimetrically using instruments selected from the group consisting of a spectrophotometer, colorimeter, spectroradiometer, densitometer, and microwell plate reader.
9 . The method of claim 6 , wherein said assay is used as a model for collagen deposition disorders selected from the group consisting of idiopathic pulmonary fibrosis, liver fibrosis, renal fibrosis, heart fibrosis, rheumatoid arthritis, and atherosclerotic plaques.
10 . The method of claim 6 , wherein said assay is used in a post-transplant patient.
11 . A collagen synthesis assay composition, comprising:
growing collagen producing cells to confluency in the presence of a candidate compound, a fixing agent, a solvent, and a collagen complexing dye;
wherein said composition is useful in determining whether said candidate compound modulates collagen synthesis; and
wherein said collagen is left intact in said cells.
12 . The method of claim 11 , wherein said cells are grown to post-confluency.
13 . The method of claim 11 , wherein said determining is done visually or colorimetrically using instruments selected from the group consisting of a spectrophotometer, colorimeter, spectroradiometer, densitometer, and microwell plate reader.
14 . The method of claim 11 , wherein said assay is used as a model for collagen deposition disorders selected from the group consisting of idiopathic pulmonary fibrosis, liver fibrosis, renal fibrosis, heart fibrosis, rheumatoid arthritis, and atherosclerotic plaques.
15 . The method of claim 11 , wherein said assay is used in a post-transplant patient.