Un-supported polymeric film with embedded microbeads
View Patent ↗The present invention relates to polymer-bead composites having a single layer planar, crystalline assembly of encoded beads embedded in a hydrophilic polymeric matrix. The composite may be unattached to a solid support. The encoded beads have different biomolecules attached to their surfaces, and the encoding permits distinguishing beads having different biomolecules attached thereto. The present invention also relates to a systematic process for the creation of functionally organized, spatially patterned assemblies of polymer-microparticle composites, including the AC electric field-mediated assembly of patterned, self-supporting organic (polymeric) films and organic-polymer-microparticle composites of tailored composition and morphology. The present invention also relates to the application of such functional assemblies in materials science and biology. Additional areas of application include sensors, catalysts, membranes, and micro-reactors, and miniaturized format for generation of multifunctional thin films.
1. A polymer-bead composite comprising: a single layer planar, crystalline assembly of encoded beads embedded in a hydrophilic polymeric matrix, wherein the composite is not attached to a solid support, and wherein differently-encoded beads have different biomolecules attached to their surfaces and wherein the encoding permits distinguishing of beads having different biomolecules attached thereto from each other.
2. The polymer-bead composite of claim 1 wherein the beads are encoded with a unique chemical or physical characteristic.
3. The polymer-bead composite of claim 2 wherein the encoding is with a fluorescent dye.
4. The polymer-bead composite of claim 1 wherein the biomolecules are peptides, proteins, nucleic acids, oligonucleotide, ligands or receptors.
5. The polymer-bead composite of claim 1 wherein the hydrophilic polymeric matrix is alkylacrylamide or hydroxyacrylalkylate hydrogel.
6. The polymer-bead composite of claim 1 wherein the beads are magnetic beads.
7. The polymer-bead composite of claim 1 , wherein the beads are assembled in a hexagonally crystalline configuration.
8. The polymer-bead composite of claim 1 , wherein the beads are assembled in a bubble raft arrangement.