Reversible terminator nucleotides and methods of use
View Patent ↗Disclosed herein a reversible terminator nucleotides and methods of use.
1. A nucleoside comprising a base and a sugar in which a phosphate moiety label is attached to said base and a phosphate moiety protecting group is attached to said sugar, wherein said phosphate moiety label and said phosphate moiety protecting group are detachable from said nucleoside by an enzyme.
2. A nucleoside comprising a base and a sugar in which a SO 3 moiety label is attached to said base and a SO 3 moiety protecting group is attached to said sugar, wherein said SO 3 moiety label and said SO 3 moiety protecting group are detachable from said nucleoside by an enzyme.
3. A nucleotide comprising a base and a sugar in which a phosphate moiety label is attached to said base and a phosphate moiety protecting group is attached to said sugar, wherein said phosphate moiety label and said phosphate moiety protecting group are detachable from said nucleotide by an enzyme.
4. A nucleotide comprising a base and a sugar in which a SO 3 moiety label is attached to said base and a SO 3 moiety protecting group is attached to said sugar, wherein said SO 3 moiety label and said SO 3 moiety protecting group are detachable from said nucleotide by an enzyme.
5. A nucleotide comprising a base and a sugar in which a label is attached to said base and a protecting group is attached to said sugar, wherein said label and said protecting group are detachable from said nucleotide by a phosphatase enzyme.
6. A nucleotide comprising a base and a sugar in which a label is attached to said base and a protecting group is attached to said sugar, wherein said label and said protecting group are detachable from said nucleotide by a shrimp alkaline phosphatase enzyme.