Device, kit and method for hookworm antigen capture and detection
A device, kit and method for detecting the presence or absence of hookworm antigens. The device, kit and method of the present invention may be used to confirm the presence or absence of hookworm in a fecal sample that may be infected with one or more of roundworm, whipworm, tapeworm and heartworm, and whether or not hookworm ova are present in the sample. Further, the device, kit and method of the present invention may be used to confirm the presence or absence of hookworm in a fecal sample excreted by a canine animal as early as nine days after the animal first becomes infected with hookworm.
1. A method of detecting the presence or absence of hookworm antigen in a fecal sample of a mammal comprising:
(a) contacting the sample with one or more antibodies specific for Ancylostoma caninum Ancylostoma -secreted protein-5 (ASP5); and
(b) detecting the presence or absence of one or more hookworm antigens or one or more hookworm antibody/antigen complexes.
2. The method of claim 1 wherein the one or more antibodies are polyclonal.
3. The method of claim 1 wherein the fecal sample is substantially free of hookworm ova based on microscopic observation, and the fecal sample is excreted by the mammal after the mammal is infected with hookworm.
4. The method of claim 1 wherein the fecal sample is excreted by the mammal no more than 13 days after the mammal has become infected with hookworm.
5. The method of claim 1 wherein the one or more antibodies specific for Ancylostoma caninum ASP5 are not capable of detecting any coproantigen derived from the group consisting of roundworm, whipworm, tapeworm and heartworm.
6. The method of claim 1 wherein the detecting the presence or absence of one or more antibody/antigen complexes includes the step of providing a secondary antibody that binds to the one or more antibody/antigen complexes.
7. The method of claim 6 wherein the secondary antibody is labeled.
8. The method of claim 1 wherein one or more of the one or more antibodies are labeled.
9. The method of claim 1 wherein the one or more antibodies are immobilized on a solid support.
10. The method of claim 9 wherein the solid support forms part of an enzyme-linked immunosorbent assay device.
11. The method of claim 10 wherein the enzyme-linked immunosorbent assay device is a lateral flow immunoassay device.
12. The method of claim 1 further comprising the step of applying the sample to one or more reagents to detect one or more of the group consisting of: one or more non-hookworm worm parasites, one or more non-worm parasites, one or more viruses, and one or more bacteria.
13. The method of claim 12 wherein the reagents for the detection of any one or all of the one or more non-hookworm worm parasites, the one or more non-worm parasites, the one or more viruses and the one or more bacteria are one or more antibodies or one or more antigens recognized by antibodies specific for the one or more non-hookworm worm parasites, the one or more non-worm parasites, the one or more viruses or the one or more bacteria.