Antibodies to IL-17A and uses thereof
Binding members, especially antibody molecules, for interleukin 17 (IL-17). The binding members are useful for the treatment of disorders associated with interleukin 17 such as rheumatoid arthritis.
1. An isolated antibody or antibody fragment thereof for human IL-17A, wherein the antibody or antibody fragment thereof comprises a set of CDRs comprising HCDR1, HCDR2 and HCDR3, which amino acid sequences are identical to SEQ ID NOs:63, 64 and 65, respectively; and LCDR1, LCDR2 and LCDR3, which amino acid sequences are identical to SEQ ID NOs:68, 69 and 70, respectively.
2. The antibody or antibody fragment thereof according to claim 1 , wherein the antibody or antibody fragment thereof has an IC50 of not more than 1 nM as determined in an HT1080 cell assay of IL-6 release in response to 1 nM human IL-17A.
3. The antibody or antibody fragment thereof according to claim 2 , wherein the antibody or antibody fragment thereof has an IC50 of not more than 0.5 nM as determined in an HT1080 cell assay of synergised release of IL-6 in response to 125 pM human IL-17A and 25 pM TNFα.
4. An isolated antibody or antibody fragment thereof for human IL-17A, comprising a VH domain with the VH domain amino acid sequence shown in SEQ ID NO: 62 and a VL domain with the VL domain amino acid sequence shown in SEQ ID NO: 176.
5. The antibody or antibody fragment thereof according to claim 4 , which is an IgG1.
6. The antibody or antibody fragment thereof according to claim 4 , which is an scFv.
7. A composition comprising the antibody or antibody fragment thereof of claim 4 and a pharmaceutically acceptable excipient.
8. An isolated antibody or antibody fragment thereof for human IL-17A, wherein the antibody or antibody fragment thereof comprises a set of CDRs comprising HCDR1, HCDR2, HCDR3, LCDR1, LCDR2 and LCDR3, which amino acid sequences are selected from the group consisting of:
(a) SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5; SEQ ID NO:8, SEQ ID NO:9 and SEQ ID NO:10;
(b) SEQ IDNO:13, SEQ IDNO:14, SEQ IDNO:15; SEQ IDNO:18, SEQ IDNO:19 and SEQ ID NO:20; and
(c) SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25; SEQ ID NO:28, SEQ ID NO:29 and SEQ ID NO:30.