Plastidic phosphoglucomutase genes
This invention relates to an isolated nucleic acid fragment encoding a plastidic phosphoglucomutase protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the plastidic phosphoglucomutase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the plastidic phosphoglucomutase in a transformed host cell.
1. An isolated polynucleotide comprising:
(a) a nucleotide sequence encoding a polypeptide having phosphoglucomutase activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:4 have at least 95% sequence identity based on the Clustal alignment method with pairwise alignment default parameters of KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, further wherein the amino acid sequence is 605 amino acids long; or
(b) the full-length complement of the nucleotide sequence of (a).
2. The isolated polynucleotide of claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:4.
3. The isolated polynucleotide of claim 1 , wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO:3.
4. A recombinant DNA construct comprising the polynucleotide of claim 1 operably linked to a regulatory sequence.
5. A vector comprising the polynucleotide of claim 1 .
6. A method for transforming a cell comprising transforming a cell with the polynucleotide of claim 1 .
7. A cell comprising the recombinant DNA construct of claim 4 .
8. A method for producing a transgenic plant comprising transforming a plant cell with the recombinant DNA construct of claim 4 and regenerating a transgenic plant from the transformed plant cell.
9. A plant comprising the recombinant DNA construct of claim 4 .
10. A seed comprising the recombinant DNA construct of claim 4 .
11. A method for positive selection of a transformed cell comprising:
(a) transforming a host cell with the recombinant DNA construct of claim 4 ; and
(b) growing the transformed host cell under conditions which allow expression of a polynucleotide in an amount sufficient to complement a null mutant to provide a positive selection means.
12. The method of claim 11 wherein the host cell is a plant.
13. The method of claim 12 wherein the plant cell is a monocot.
14. The method of claim 12 wherein the plant cell is a dicot.
15. A method of altering the level of expression of a plastidic phosphoglucomutase protein in a host cell comprising:
(a) transforming a host cell with the recombinant DNA construct of claim 4 ; and
(b) growing the transformed host cell produced in step (a) under conditions that are suitable for expression of the recombinant DNA construct wherein expression of the recombinant DNA construct results in production of altered levels of a plastidic phosphoglucomutase polypeptide in the transformed host cell.