Method of production of L-amino acids
View Patent ↗An isolated polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, with the L-aspartic acid at position 5 of the amino acid sequence replaced by another proteinogenic amino acid, and possesses citrate synthase activity. In addition, a vector comprises the polynucleotide and a bacterium comprises the vector. An isolated polynucleotide comprises a nucleotide sequence comprising, from position 1 to 39, the nucleotide sequence corresponding to position 1 to 39 of SEQ ID NO: 11, from position 40 to 105, a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 12, with each proteinogenic amino acid except L-aspartic acid being present at position 5. A method of producing an L-amino acids is also described.
1. An isolated polynucleotide, encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, wherein L-aspartic acid at position 5 of the amino acid sequence is replaced by L-valine, and wherein the polypeptide possesses a citrate synthase activity.
2. The isolated polynucleotide of claim 1 , wherein the isolated polynucleotide comprises the nucleotide sequence of SEQ ID NO: 5.
3. A vector comprising the polynucleotide as claimed in claim 1 .
4. A bacterium that has been transformed with the vector as claimed in claim 3 , wherein the bacterium is Corynebacterium glutamicum.
5. A bacterium of Corynebacterium glutamicum , which comprises the polynucleotide as claimed in claim 1 .
6. A recombinant bacterium of Corynebacterium glutamicum comprising a polynucleotide that codes for a polypeptide possessing a citrate synthase activity, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 2 in which L-aspartic acid at position 5 is substituted with L-valine.
7. The recombinant bacterium as claimed in claim 6 , wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO: 5.