Method for production of arachidonic acid
View Patent ↗The present invention relates to a process for producing arachidonic acid. In one embodiment, Mortierella sect. schmuckeri microorganisms are cultured in fermentation medium, preferably containing a component of a complex nitrogen source. Further disclosed is a food product which includes Mortierella sect. schmuckeri microorganisms or lipid isolated from such microorganisms to enhance the arachidonic acid content of the food product.
1. A method for extending the shelf life of Mortierella sect. schmuckeri cells or arachidonic acid rich oil from cells of Mortierella sect. schmuckeri , comprising storing the oil or cells under non-oxidizing conditions; wherein said Mortierella sect. schmuckeri produces at least about 0.70 grams of arachidonic acid per liter per day when grown under appropriate fermentation conditions.
2. The method of claim 1 , wherein the non-oxidizing conditions comprise a vacuum.
3. The method of claim 1 , wherein the non-oxidizing conditions comprise a non-oxidizing atmosphere.
4. The method of claim 1 , wherein the non-oxidizing atmosphere is N 2 or CO 2 .
5. The method of claim 1 , wherein said Mortierella sect. schmuckeri is of the species Mortierella schmuckeri.
6. The method of claim 1 , wherein said Mortierella sect. schmuckeri is of the species Mortierella camargensis.
7. The method of claim 1 , wherein said Mortierella sect. schmuckeri produces at least about 0.80 grams of arachidonic acid per liter per day when grown under appropriate fermentation conditions.
8. The method of claim 1 , wherein said Mortierella sect. schmuckeri produces at least about 0.86 grams of arachidonic acid per liter per day when grown under appropriate fermentation conditions.
9. The method of claim 1 , wherein said Mortierella sect. schmuckeri grows as a dispersed filamentous form when grown under liquid culture conditions.
10. The method of claim 1 , wherein at least about 21.9% of the total fatty acid of said Mortierella sect. schmuckeri is arachidonic acid.