Method for targeting lysosomal enzymes
View Patent ↗Targeted therapeutics that localize to a specific subcellular compartment such as the lysosome are provided. The targeted therapeutics include a therapeutic agent and a targeting moiety that binds a receptor on an exterior surface of the cell, permitting proper subcellular localization of the targeted therapeutic upon internalization of the receptor. Nucleic acids, cells, and methods relating to the practice of the invention are also provided.
1. A method of targeting a therapeutic enzyme to a lysosome, the method comprising:
(a) providing a targeted therapeutic comprising a therapeutic enzyme and a lysosomal-targeting domain that binds the cation-independent mannose-6-phosphate receptor in a mannose-6-phosphate-independent manner; wherein the lysosomal-targeting domain comprises a mature human IGF-II;
(b) contacting the targeted therapeutic with a cell deficient in a lysosomal enzyme, wherein the cell expresses the cation-independent mannose-6-phosphate receptor on the surface, thereby targeting the therapeutic enzyme to the lysosome as a result of the binding between the lysosomal-targeting domain and the cation-independent mannose-6-phosphate receptor.
2. The method of claim 1 , wherein the therapeutic enzyme is a lysosomal enzyme.
3. The method of claim 2 , wherein the lysosomal enzyme is human acid-α1,4-glucosidase.
4. A method of targeting a therapeutic enzyme to a lysosome, the method comprising:
a) providing a targeted therapeutic comprising a therapeutic enzyme and a lysosomal-targeting domain that binds the cation-independent mannose-6-phosphate receptor in a mannose-6-phosphate-independent manner; wherein the lysosomal-targeting domain comprises a mutein of mature human IGF-II having an amino acid sequence at least 70% identical to mature human IGF-II;
b) contacting the targeted therapeutic with a cell deficient in a lysosomal enzyme, wherein the cell expresses the cation-independent mannose-6-phosphate receptor on the surface,
thereby targeting the therapeutic enzyme to the lysosome as a result of the binding between the lysosomal-targeting domain and the cation-independent mannose-6-phosphate receptor.
5. The method of claim 4 , wherein the mutein comprises a deletion or a replacement of amino acids 1-7 of mature human IGF-II.
6. The method of claim 5 , wherein the mutein comprises a replacement of amino acids 1-7 of mature human IGF-II with one or more amino acid residues.
7. The method of claim 4 , wherein the mutein is a fragment of mature human IGF-II.
8. The method of claim 4 , wherein the therapeutic enzyme is a lysosomal enzyme.
9. The method of claim 8 , wherein the lysosomal enzyme is human acid-α1,4-glucosidase.
10. A method of treating a lysosomal storage disease comprising administering to a subject in need of treatment a targeted therapeutic comprising a lysosomal enzyme and a lysosomal-targeting domain that binds the cation-independent mannose-6-phosphate receptor in a mannose-6-phosphate-independent manner, wherein the lysosomal-targeting domain comprises a mature human IGF-II.
11. The method of claim 10 , wherein the lysosomal enzyme is human acid-α1,4-glucosidase.
12. A method of treating a lysosomal storage disease comprising administering to a subject in need of treatment a targeted therapeutic comprising a lysosomal enzyme and a lysosomal-targeting domain that binds the cation-independent mannose-6-phosphate receptor in a mannose-6-phosphate-independent manner, wherein the lysosomal-targeting domain comprises a mutein of mature human IGF-II having an amino acid sequence at least 70% identical to mature human IGF-II.
13. The method of claim 12 , wherein the mutein comprises a deletion or a replacement of amino acids 1-7 of mature human IGF-II.
14. The method of claim 13 , wherein the mutein comprises a replacement of amino acids 1-7 of mature human IGF-II with one or more amino acid residues.
15. The method of claim 12 , wherein the lysosomal enzyme is human acid-α1,4-glucosidase.